alexa The Pro-Inflammatory Cytokines Expression of Porcine Embryonic Stem Cells Xenotransplanted into the Brain and Spinal Cord in Rats
ISSN: 2157-7013

Journal of Cell Science & Therapy
Open Access

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Research Article

The Pro-Inflammatory Cytokines Expression of Porcine Embryonic Stem Cells Xenotransplanted into the Brain and Spinal Cord in Rats

Chia-Hsin Liao1,#, Yu-Jing Liao2,#, Kuo Yuan3,4,5,#, Yu-Chi Yang3, Yu-Yu Joyce Ho1, Jiunn-Wang Liao6, Lih-Ren Chen2,7,8, Yow-Ling Shiue9 and Jenn-Rong Yang2*

1Department of Medical Research, Buddhist Tzu Chi General Hospital, Hualien, Taiwan

2Division of Physiology, Livestock Research Institute, Council of Agriculture Executive Yuan, Tainan, Taiwan

3Department of Oral Medicine, National Cheng Kung University Hospital, Tainan, Taiwan

4Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan

5School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan

6Graduate Institute of Veterinary Pathobiology, National Chung Hsing University, Taichung, Taiwan

7Institute of Biotechnology, National Cheng Kung University, Tainan, Taiwan

8Institute of Biotechnology, Southern Taiwan University, Tainan, Taiwan

9Institute of Biomedical Science, National Sun Yat-Sen University, Kaohsiung, Taiwan

#These authors have contributed Equally.

*Corresponding Author:
Jenn-Rong Yang
Physiology Division, Livestock Research Institute
Council of Agriculture, Executive Yuan, Tainan, Taiwan. 112
Farm Rd., Hsinhua, Tainan, Taiwan
Tel: +886-6-5911884
Fax: +886-6-5912581
E-mail: [email protected]

Received Date: May 15, 2014; Accepted Date: Jun 25, 2014; Published Date: Jun 27, 2014

Citation: Liao CH, Liao YJ, Yuan K, Yang YC, Ho YYJ, et al. (2014) The Pro-Inflammatory Cytokines Expression of Porcine Embryonic Stem Cells Xenotransplanted into the Brain and Spinal Cord in Rats. J Cell Sci Ther 5:168. doi: 10.4172/2157-7013.1000168

Copyright: © 2014 Liao CH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



In this study, we transplanted green fluorescent protein-expressing porcine embryonic stem (pES/GFP+) cells and their derived cells, D12 neuronal progenitors (D12 NP) and D18 neuronal progenitors (D18 NP), into the brain and spinal cord of Sprague-Dawley (SD) rats to investigate the differences of inflammation after xenotransplantation. On day 3, 7, and 14 after transplantation, we collected the brain and spinal cord tissues to investigate the gene expression of interleukin 1-α and -β (IL-1α and IL-1β), interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α). One month after transplantation, histopathological changes of the brain and spinal cord were examined by H&E staining. The expression patterns of inflammatory-related factors in the spinal cord were more dramatic than those in the brain. On day 14 after transplantation, only D18 NP significantly elevated the expressions of IL-1α and IL-1β in the brain, but almost all grafted cells evoked the expression of IL-1α, IL-1β, IL-6, and TNF-α in the spinal cord. Following H&E staining, no dramatic histopathological abnormality was revealed, indicating that no acute damage can be observed following the experimental period albeit inflammatory gene expressions were temporarily triggered. Although the gene expression patterns varied during the experimental period, no lethality appeared in the rats after xenotransplantation of pES/GFP+ cells. These indicate that pES/GFP+ cells may be a safe cell resource for future application on regenerative medicine.

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