The Quest for More Tolerant Rice: How High Concentrations of Iron Affect Alternative Splicing?
|Artur Teixeira de Araujo Junior1, Daniel da Rosa Farias1, Railson Schreinert dos Santos1, Marcelo Nogueira do Amaral3, Luis Willian Pacheco Arge1, Danyela de Cássia Oliveira1, Solange Ferreira da Silveira Silveira1, Rogério Oliveira de Sousa2, Eugenia Jacira Bolacel Braga3, Luciano Carlos da Maia1 and Antonio Costa de Oliveira1*|
|1Plant Genomics and Breeding Center, Universidade Federal de Pelotas, Campus Capão do Leão, Brazil|
|2Department of Soils, Universidade Federal de Pelotas, Campus Capão do Leão, Brazil|
|3Department of Botany, Universidade Federal de Pelotas, Campus Capão do Leão, Brazil|
|Corresponding Author :||Antonio Costa de Oliveira
Plant Genomics and Breeding Center
Universidade Federal de Pelotas, Campus Capão do Leão
96010-900 Capão do Leão, Brazil
E-mail: [email protected]
|Received November 05, 2015; Accepted December 05, 2015; Published December 09, 2015|
|Citation: Junior ATD, Farias DD, dos Santos RS, do Amaral MN, Arge LWP, et al. (2015) The Quest for More Tolerant Rice: How High Concentrations of Iron Affect Alternative Splicing? Transcriptomics 3:122. doi:10.4172/2329-8936.1000122|
|Copyright: © 2015 Junior ATD et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
|Related article at Pubmed, Scholar Google|
Rice (Oryza sativa L.) is a global staple food crop and an important model organism for plant studies. Recent reports have shown that alternative splicing is affected by many stressful conditions, suggesting its importance for the adaptation to adverse environments. Due to the little information on this subject, this study aimed to explore changes in splicing patterns that occur in response to high iron concentration in nutrient solutions. Here we quantified different kind of junctions and splicing events in the transcriptome of a relatively tolerant rice cultivar BRS Querência, under iron excess with concentration of 300 mg L-1 Fe+2. Plants kept under standard conditions (control) presented 127,781 different splicing junctions, while stressed plants had 123,682 different junctions. Canonical (98.85% and 98.91%), semi-canonical (0.73% and 0.70%) and non-canonical (0.42% and 0.40%) junctions were found in control and stressed plants, respectively. Intron retention was the most frequent event (44.1% and 47.4%), followed by 3’ splice site (22.6% and 21.9%), exon skipping (18.9% and 17.3%) and alternative 5’ splice site (14.4% and 13.4%) in control and stressed plants, respectively. We also found 25 differentially expressed genes (five up and 20 down regulated) that are related to post-translational modifications. These results represent an important step in the understanding of how plant stress responses occur in an iron tolerant genotype, uncovering novel genes involved in iron stress response.