The Relationship between Bioactive Compounds with Diastase Activity and Antibacterial Synergy of Honey and Potato Starch Combinations against Klebsiella pneumoniaMoussa Ahmed*, Baghdad Khiati, Saad Aissat Noureddine Djebli, Abdelmalek Meslem and Salima Bacha
Pharmacognosy and Api-Phytotherapy Research Laboratory, Mostaganem University, Algeria
- *Corresponding Author:
- Moussa Ahmed
Pharmacognosy and Api-Phytotherapy Research Laboratory
Mostaganem University, Algeria
Tel: +213 65234059
Fax: +213 65234059
E-mail: [email protected]
Received date: July 22, 2013; Accepted date: October 17, 2013; Published date: October 22, 2013
Citation: Ahmed M, Khiati B, Aissat S, Djebli N, Meslem A, et al. (2013) The Relationship between Bioactive Compounds with Diastase Activity and Antibacterial Synergy of Honey and Potato Starch Combinations against Klebsiella pneumonia. Clin Microbial 2:131. doi: 10.4172/2327-5073.1000131
Copyright: © 2013 Ahmed M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Honey samples produced by Apismellifera, both unifloral and multifloral from different sources in Algeria were examined for their antibacterial capacity and their synergism with potato starch. An agar incorporation technique was used to assess the minimum inhibition concentration (MIC) and the minimum inhibition additive concentration (MIAC) of honey against K. pneumoniae ATCC 27736. The physicochemical properties, α-amylases activity, total phenolic and flavonoid content, of six representative honey samples were determined. The MIC for the six varieties of honey without potato starch against K. pneumoniae ranged between 14% and 24% (v ⁄ v). When starch was incubated with honey and then added to media, an MIC drop was noticed with each variety and it ranged between 5.55 % and 16.66%.The total phenolic content of honey samples was between 1.50–108.21 mg GAE/100 g honey as gallic acid equivalent, total flavonoid content varied from 5.41 to 9.94 mg Catechin/kg . Mean value for diastase was 16.55 ± 2.8 (range 7.3–23.5) expressed as diastase number in Gothe’sscale. No significant correlation was established between α-amylases activity and bioactive compounds. Honey: potato starch combinations show real potential for future use as a treatment for infections caused by K. pneumonia.