The results of clinic-morphological investigation of glutamic acid influence on the experimental dental fluorosis (The Experimental research)
Objective. The experimental investigations on the teeth of rats for determining the prophylaxis influence of the glutamic acid on the level of dental (teeth) fluorosis were made. Have been studied both clinical and histological conditions (picture) of teeth of experimental animals. Methods We made experiment on 60 rats with 30-50 gr by weight. The animals have been divided into 3 groups 20 rats in each group. The I groups (control) the rats which were on a usual vivarium ration and received water with the contents of fluorine 0,30-0,45 mg/l; II group (comparision) rats receiving water with the contents of fluorine 15 mg/l; III group ? (main group) ? the animals received both water with the contents of fluorine 15 mg /l and 1% solution of glutamic acid 2 times per a week intra orally. Histological investigations have been made on sections of rat?s teeth, which were painted (colored) with hemotoxilin ?eozine, thionine. Results. On incisors of rats receiving water with the contents 15 mg/l of fluorine at 4-5 weeks the area (zone) of depigmentation characteristic for dental fluorosis has appeared. Microscopically was observed hypoplasia, enamel prisms have both crimped and indirect courses. The results of these were the infringement of Sroger line and sometimes its abcence. In group of rats receiving simultaneously water with high concentrations of fluorine and 1% solution of glutamic acid similar both clinical and hystochemikal changes were not occurred (marked). Conclusion. The glutamic acid prevented teeth fluorosis because of neutralization of a fluoric intoxiation (high level of fluorine).