alexa The Role of 16s rDNA PCR in the Diagnosis of Peritoneal Dialysis-Associated Peritonitis
ISSN: 2161-0703

Journal of Medical Microbiology & Diagnosis
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

The Role of 16s rDNA PCR in the Diagnosis of Peritoneal Dialysis-Associated Peritonitis

Holly L. Ciesielczuk1, Robert J. Shorten1, Andrew Davenport2, Hala Kandil1, Jane E. Carpenter3, Timothy D. McHugh4 and Sophie E. Collier1*

1Department of Microbiology, Royal Free London NHS Foundation Trust, London, United Kingdom

2Department of Nephrology, Royal Free London NHS Foundation Trust, London, United Kingdom

3Department of Microbiology, West Middlesex University, Isleworth, United Kingdom

4Centre of Clinical Microbiology, Department of Infection and Immunity, University College London, United Kingdom

*Corresponding Author:
Sophie Collier
Department of Microbiology
Royal Free Hospital, Pond Street
London NW3 2QG, United Kingdom
Tel: 0207 794 0500 #38282
E-mail: [email protected]

Received date: September 27, 2012; Accepted date: October 19, 2012; Published date: October 22, 2012

Citation: Ciesielczuk HL, Shorten RJ, Davenport A, Kandil H, Carpenter JE, McHugh TD, et al. (2012) The Role of 16s rDNA PCR in the Diagnosis of Peritoneal Dialysis- Associated Peritonitis. J Med Microb Diagn 1:116. doi:10.4172/2161-0703.1000116

Copyright: © 2012 Collier SE, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

 Introduction: Despite recent advances in Peritoneal Dialysis (PD), peritonitis remains the most common and serious complication. In a significant proportion of patients a pathogen is not cultured. In this study we investigated the use of 16S rDNA PCR to make a bacterial diagnosis.
Methods: We used an optimised DNA extraction and 16S rDNA PCR with DNA sequencing to detect pathogens in Peritoneal Dialysis-Associated Peritonitis (PDAP).
Results: Seventy-one PD fluids from twenty-four patients were analysed. In suspected cases of PDAP, thirteen out of twenty-one patients had a bacterial pathogen cultured and 16S rDNA PCR with DNA sequencing identified one additional pathogen. However 16S rDNA PCR only detected the pathogen in five of the culture-positive fluids. All follow-up samples were culture-negative, but possible pathogens were identified in three samples by the 16S rDNA PCR.In suspected cases of PDAP the sensitivity and specificity of the PCR was calculated as 69% and 63%, respectively. The negative predictive value of the PCR in follow-up fluids was 100%.
Conclusion: The use of 16S rDNA PCR in diagnosis of PDAP needs further study and improved sensitivity before widespread introduction.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords