The Role of 16s rDNA PCR in the Diagnosis of Peritoneal Dialysis-Associated PeritonitisHolly L. Ciesielczuk1, Robert J. Shorten1, Andrew Davenport2, Hala Kandil1, Jane E. Carpenter3, Timothy D. McHugh4 and Sophie E. Collier1*
- *Corresponding Author:
- Sophie Collier
Department of Microbiology
Royal Free Hospital, Pond Street
London NW3 2QG, United Kingdom
Tel: 0207 794 0500 #38282
E-mail: [email protected]
Received date: September 27, 2012; Accepted date: October 19, 2012; Published date: October 22, 2012
Citation: Ciesielczuk HL, Shorten RJ, Davenport A, Kandil H, Carpenter JE, McHugh TD, et al. (2012) The Role of 16s rDNA PCR in the Diagnosis of Peritoneal Dialysis- Associated Peritonitis. J Med Microb Diagn 1:116. doi:10.4172/2161-0703.1000116
Copyright: © 2012 Collier SE, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Introduction: Despite recent advances in Peritoneal Dialysis (PD), peritonitis remains the most common and serious complication. In a significant proportion of patients a pathogen is not cultured. In this study we investigated the use of 16S rDNA PCR to make a bacterial diagnosis.
Methods: We used an optimised DNA extraction and 16S rDNA PCR with DNA sequencing to detect pathogens in Peritoneal Dialysis-Associated Peritonitis (PDAP).
Results: Seventy-one PD fluids from twenty-four patients were analysed. In suspected cases of PDAP, thirteen out of twenty-one patients had a bacterial pathogen cultured and 16S rDNA PCR with DNA sequencing identified one additional pathogen. However 16S rDNA PCR only detected the pathogen in five of the culture-positive fluids. All follow-up samples were culture-negative, but possible pathogens were identified in three samples by the 16S rDNA PCR.In suspected cases of PDAP the sensitivity and specificity of the PCR was calculated as 69% and 63%, respectively. The negative predictive value of the PCR in follow-up fluids was 100%.
Conclusion: The use of 16S rDNA PCR in diagnosis of PDAP needs further study and improved sensitivity before widespread introduction.