alexa The Role of Combined Nicotine and Caffeine Administrati
ISSN: 2157-7099

Journal of Cytology & Histology
Open Access

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Research Article

The Role of Combined Nicotine and Caffeine Administration on the Histological Structure of the Rat Urinary Bladder with a Special Reference to P53

Alhan I Allehaibi1, Siham K Abunasef1,2* and Azza I Zaki1,3

1Anatomy Department, Faculty of Medicine, King Abdulaziz University, Saudi Arabia

2Histology Department, Faculty of Medicine, Ain Shams University, Egypt

3Anatomy Department, Faculty of Medicine, Alexandria University, Egypt

*Corresponding Author:
Abunasef SK
Anatomy Department
Faculty of Medicine, King Abdulaziz University
Saudi Arabia
Tel: 00966543561012
E-mail: [email protected]

Received Date: November 21, 2015; Accepted Date: June 20, 2017; Published Date: June 28, 2017

Citation: Allehaibi AI, Abunasef SK, Zaki AI (2017) The Role of Combined Nicotine and Caffeine Administration on the Histological Structure of the Rat Urinary Bladder with a Special Reference to P53. J Cytol Histol 8: 455. doi: 10.4172/2157- 7099.1000455

Copyright: © 2017 Allehaibi AI, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Background: It is difficult to find microscopic studies that can demonstrate the effect of combined administration of nicotine and caffeine on the mucosa of the rat urinary bladder. Aim of work: So, the current study aimed at demonstration of histological and p53 immuno-histochemical expression of the mucosa of rat urinary bladder as a marker sharing in process of apoptosis following the combined administration of nicotine and caffeine. Materials and methods: The animals were divided randomly into 4 groups: control, Nicotine, caffeine and combined Nicotine and Caffeine. The urinary bladder was processed and stained with hematoxylin and eosin stain, histochemically by PAS and immunohistochemically by Anti p53 antibodies. Results: After nicotine there was apparent increase in the layers of epithelium with intraepithelial lymphocytes. The strong PAS positive reaction of the basal layer together with P53 positive expression of few lamina propria cells was evident. After caffeine, the epithelial layers and mononuclear cells of the lamina propria were increased. There was a marked decrease in the PAS positive reacting cells of the superficial cell layer. The groups that have administered combine caffeine and nicotine; there were focal areas of epithelial erosion with proliferating basal layers. There was a strong PAS positive reaction under the basal layer and p53 positive expression of many lamina propria cells. Conclusion: The present study had concluded that the chronic administration of combined nicotine and caffeine could result in epithelial hyperplasia associated with positive P53 expression in nuclei of lamina propria cells of the urinary bladder mucosa.


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