alexa The Role of DNA Damage and Repair Proteins in Adipose-Derived Adult Stem Cell Differentiation in Neural- Like Cells | OMICS International
ISSN: 2157-7552

Journal of Tissue Science & Engineering
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Research Article

The Role of DNA Damage and Repair Proteins in Adipose-Derived Adult Stem Cell Differentiation in Neural- Like Cells

Ana Paula Franco Lambert1, Dinara Moura2, Aline Fraga Zandonai3, Mariana Assis Lemos4, Jeremiah Lubianca5, Christian Viezzer6, Joao Alvaro Souza da Silva7, Diego Bonatto8, Denise Cantarelli Machado9 and Joao Antonio Pegas Henriques10*

1Ana Paula Franco Lambert, Departamento de Biofísica/Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil and Instituto de Biotecnologia, Universidade de Caxias do Sul (UCS), Caxias do Sul, RS, Brazil

2Dinara Moura, Departamento de Biofísica/Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil

3Aline Fraga Zandonai, Departamento de Biofísica/Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil

4Mariana Assis Lemos, Departamento de Medicina Interna/Faculdade de Medicina, Centro de Terapia Celular-Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil

5Jeremiah Lubianca, Departamento de Medicina Interna/Faculdade de Medicina, Centro de Terapia Celular-Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil

6Christian Viezzer, Departamento de Medicina Interna/Faculdade de Medicina, Centro de Terapia Celular-Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil

7João Álvaro Souza da Silva, Departamento de Medicina Interna/Faculdade de Medicina, Centro de Terapia Celular-Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil

8Diego Bonatto, Departamento de Biofísica/Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil

9Denise Cantarelli Machado, Departamento de Medicina Interna/Faculdade de Medicina, Centro de Terapia Celular-Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil

10Joao Antônio Pêgas Henriques, Departamento de Biofísica/Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil and Instituto de Biotecnologia, Universidade de Caxias do Sul (UCS), Caxias do Sul, RS, Brazil

Corresponding Author:
Dr. Joao Antonio Pegas Henriques
Departamen to de Biofísica/Centro de Biotecnologia
UFRGS, Av. Bento Gonçalves 9500
Porto Alegre, RS, Brazil, 91507-970
Tel: 55-51-3308-7602
Fax: 55-51-3308-6084
E-mail: [email protected], [email protected]

Received date: September 03, 2011; Accepted date: November 15, 2011; Published date: November 17, 2011

Citation: Lambert APF, Moura D, Zandonai AF, Lemos MA, Lubianca J, et al. (2011) The Role of DNA Damage and Repair Proteins in Adipose-Derived Adult Stem Cell Differentiation in Neural- Like Cells. J Tissue Sci Eng 2:109. doi:10.4172/2157-7552.1000109

Copyright: © 2011 Lambert APF, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The development of a clinically translatable method of engineering with adipose-derived adult stem (ADAS) for reconstruction requires investigation of several components. The differentiation of ADAS cells into neuronal cells has been reported by several groups. The stringent maintenance of genomic stability in adult stem cells via anti-stress defenses and DNA repair mechanisms is particularly important because any genetic alteration can compromise the genomic stability and functionality of the cell. The main objective of this data was to examine some parameters related to DNA damage in cells submitted to the neural differentiation protocol and to understand if DNA damage can be associated to cell differentiation. The comet assay, micronucleus tests, and the cell viability assay were utilized to observe ADAS cells treated with neural induction medium. The results of our genotoxicity assays suggest that increased DNA damage observable by the comet assay was induced by neural differentiation. Emerging findings suggest that DNA damage; telomerase and DNA repair proteins play important roles in neurogenesis developing. Surprisingly we obtain evidence for an association between DNA damage and neuronal-like differentiation and hypothesize that during neural differentiation DNA damage will recruit telomerase TIP60 and MCM3, where they may function in DNA repair, chromatin remodeling and limiting DNA replication.

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