The Role of TREM-1 Gene Expression and Soluble TREM-1 as Prognostic Markers of Sepsis
- *Corresponding Author:
- Dina M. Atef
Clinical Pathology Department
Faculty of Medicine
Zagazig University, Egypt
E-mail: [email protected]
Received Date: September 10, 2015; Accepted Date: December 07, 2015; Published Date: December 09, 2015
Citation: Atef DM, Ghonaim RA, Nada EN (2015) The Role of TREM-1 Gene Expression and Soluble TREM-1 as Prognostic Markers of Sepsis. J Med Microb Diagn 5:211. doi: 10.4172/2161-0703.1000211
Copyright: © 2015 Atef DM, et al.. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Determination of TREM-1 expression on monocytes has been investigated as a perspective diagnostic method to distinguish infectious from non-infectious etiology of the inflammation.
Objectives: To analyse the expression of the TREM-1 gene in patients of sepsis and septic shock and its relation with the severity of pathophysiological conditions. Also to describe the dynamics changes of soluble TREM- 1, procalcitonin and C-reactive protein during the course of sepsis and Correlation between their expressions and the clinical scoring system known as APACHE II scoring system.
Material and Methods: After an ethical approval, the expression of mRNA of TREM-1 in PMNs was detected in 76 critically ill patients (49 with sepsis and 27 with septic shock) were analyzed by using quantitative real-time PCR. The concentration of s. TREM-1, CRP and PCT levels were measured by an enzyme immunoassay.
Results: On the ICU admission, the septic shock group displayed higher levels of sTREM-1, PCT, APACHE II score (P<0.001) and CRP (P=0.01) than the sepsis group. Significant positive correlation (P<0.05) between APACHE II and both sTREM-1 and PCT in the prognosis of morbidity were shown. The mRNA levels of patients suffering from sepsis and septic shock were 0.54- and 0.39-fold lower compared to those of healthy subjects (1.06) respectively.
Conclusion: With regard to sepsis diagnosis and severity, sTREM-1 and PCT have positive values in prognostic assessment of the disease and may be taken as a survival-impacting risk factor. The TREM-1 gene expression levels isolated from patients with sepsis may be used as a surrogate marker for determining the severity.