The Significance of the Lymphocytes Infiltration and Endothelialdependent Angiogenesis in Choroidal Melanomas
- *Corresponding Author:
- Joanna Reszec
Department of the Medical Pathomorphology
Medical University of Bialystok
ul. Waszyngtona 13, 15-269 Bialystok, Poland
Tel: +48 85 748 59 45
Fax: +48 85 748 59 90
E-mail: [email protected]
Received Date: October 24, 2013; Accepted Date: December 02, 2013; Published Date: December 04, 2013
Citation: Joanna R, Renata Z, Ewa PS, Lech C (2013) The Significance of the Lymphocytes Infiltration and Endothelial-dependent Angiogenesis in Choroidal Melanomas. J Cytol Histol 4:200. doi:10.4172/2157-7099.1000200
Copyright: © 2013 Joanna R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Objective: Posterior uveal melanoma is the most common primary intraocular malignant tumor in adults. Despite the clinical significant treatment, still many patients die because of unpredictable metastases. It is difficult to predict clinical outcome in individual cases of ocular melanoma basing only on the intraocular size, because of the spectrum of clinical, morphologic, and cytologic changes and lack of discrete stages.
Design: The aim of the study was to evaluate CD3, CD20 and CD34 expression within choroidal melanoma in
correlation with clinic and pathological features. Participants: 35 cases of choroidal melanoma were evaluated.
Methods: Immunohistochemical reaction was used to evaluate CD3 and CD20 (T and B cells markers) and
CD34 (endothelial cells marker) expression, with positive and negative controls. Obtained results were estimated under the light microscope.
Results: T lymphocytes were present in 94.3% choroidal melanoma specimens, the most significant CD3
expression was noticed in choroidal melanoma in pT3 stage. Presence of B lymphocytes was observed only in 22.8% cases, but only in advanced tumors (pT3). CD34 expression was observed in 77.1% choroidal melanomas. A statistically significant correlation was observed between CD34 expression and staging- 84.2% choroidal melanomas in pT3 stage were positive for CD34 expression.
Conclusions: T cell infiltration is present in both low and high advanced ocular melanomas. B cells and thin wall vessels are present mainly in advanced choroidal melanoma and due to may be associated with tumor progression.