The Stature of Human Thyroid Cancer Related to Over Expressed mir-221 and mir-146b
Jamshid Molaei, Melika Meidanchi and Sina Mirzaahmadi*
Department of the Genetics, Zanjan branch, Islamic Azad University, Zanjan, Iran
- *Corresponding Author:
- Sina Mirzaahmadi
Department of the Genetics, Zanjan branch
Islamic Azad University, Zanjan, Iran
Tel: 44865179-82, 44865154-8
E-mail: [email protected]
Received Date: Novembert 07, 2016; Accepted Date: January 22, 2017; Published Date: January 24, 2017
Citation: Molaei J, Meidanchi M, Mirzaahmadi S (2017) The Stature of Human Thyroid Cancer Related to Over Expressed mir-221 and mir-146b. J Mol Biomark Diagn S2: 030. doi:10.4172/2155-9929.S2-030
Copyright: © 2017 Molaei J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Purpose: The ability of miRNAs for biomarker tools was further built by recent research showing that cancerspecific miRNAs are traceable in cell-free blood fluids such as plasma and serum. In this study, the levels of miR-221 and miR146b in human’s peripheral blood as tumor markers for early detection of Papillary Thyroid Carcinoma [PTC] were investigated.
Methods: A stem-loop quantitative reverse transcription-polymerase chain reaction [qRT-PCR] method was performed to determine the serum levels of miR-221 and miR-146b from 25 healthy controls, 25 PTC patients the day before thyroidectomy, 25 patients with benign nodules and 25 patients after thyroidectomy during the scheduled medical examinations.
Results: There was statistical difference in the serum levels for miR-221 and miR-146b between the controls and the PTC patients. It also showed no statistical difference for miR-221 and miR-146b between controls and thyroidectomy patients.
Conclusion: The overexpression of miR-221 and miR-146b could be potentially served as serum indicator biomarkers in the approach for Thyroid Papillary Carcinoma detection from benign nodules.