alexa The Stimulative Effect of T3 and T4 on Human Myocardial
ISSN: 2155-9880

Journal of Clinical & Experimental Cardiology
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Research Article

The Stimulative Effect of T3 and T4 on Human Myocardial Endothelial Cell Proliferation, Migration and Angiogenesis

Silvana Balzan1*, Renata Del Carratore1, Caterina Nardulli1, Laura Sabatino1, Valter Lubrano2 and Giorgio Iervasi1,2
1Institute of Clinical Physiology, CNR, Via Moruzzi 1, Pisa 56124, Italy
2Fondazione CNR/Regione Toscana G. Monasterio, Via Moruzzi 1, Pisa 56124, Italy
Corresponding Author : Silvana Balzan
CNR Institute of Clinical Physiology
Via Moruzzi 1, Pisa, Italy
Tel: +39 050 3152659
Fax: +39 050 3152166
E-mail: [email protected]
Received October 04, 2013; Accepted December 24, 2013; Published December 27, 2013
Citation: Balzan S, Dell Carratore R, Nardulli C, Sabatino L, Lubrano V, et al. (2013) The Stimulative Effect of T3 and T4 on Human Myocardial Endothelial Cell Proliferation, Migration and Angiogenesis. J Clin Exp Cardiolog 4:280. doi:10.4172/2155-9880.1000280
Copyright: © 2013 Balzan S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

Angiogenesis, the process involving the growth of new blood vessels from pre-existing vessels, may be a target for combating diseases characterized by either poor vascularisation or abnormal vasculature as in cardiovascular diseases. Thyroid hormones (THs) are strong proangiogenic factors whose action starts at the plasma membrane integrin αvβ3 protein transducing rapid nongenomic signals on tumor thyroid cells. The tetraiodothyroacetic acid (Tetrac) inhibits the binding of TH to integrin receptor αVß3 blocking angiogenesis. Growing evidences suggest that, also in heart, Triiodothyronine (T3) and Thyroxine (T4) triggered nongenomic pathways through their binding to integrin receptor αVβ3 inducing capillary proliferation. The angiogenic activity of T3 and T4 has been studied by the chick choriallontoic endothelial cell microtubule assay and the human dermal microvascular endothelial cells microtubule assay. The aim of this work was to evaluate the direct stimulative activity of T3 and T4 on human cardiac microvascular endothelial cell (HMVEC-C) proliferation, migration and tube formation, employing Tetrac as inhibitor. Our in vitro study indicates that T3 and T4 directly stimulate angiogenesis in HMVEC-C observed as capillary density, cell proliferation and

In all models, Tetrac (5 μM) inhibited the proangiogenic effect of T3 and T4 suggesting its integrin-mediated action. Sq RT-PCR assay revealed that T3, and in less extent T4, increased the expression of angiogenic genes such as angiopoietin-1 (Angpt-1), angio-associated migratory cell protein (AAMP) and vascular endothelial growth factor (VEGF), whereas when the cells were pre-incubated with Tetrac this effect was abolished. In conclusion, our results show that THs stimulate angiogenesis, suggesting a potential therapeutic role aimed at increasing capillary density in cardiac diseases.

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