alexa The Study of Analytical Identification on Main Monomer
ISSN: 2157-7110

Journal of Food Processing & Technology
Open Access

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Research Article

The Study of Analytical Identification on Main Monomer Compounds of Spoiled Grass Carp by High Performance Liquid Chromatography of Quadrupole Time of Flight Mass Spectrometry

Sheng ZY, Si HJ, Ming LX, Rong CJ, Yi CZ and Hui ZY*

Sericulture and Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, China

Corresponding Author:
Hui ZY
Sericulture and Agri-food Research Institute, Guangdong Academy of Agricultural Sciences
Key Laboratory of Functional Foods, Ministry of Agriculture
Guangdong Key Laboratory of Agricultural Products Processing
Guangzhou 510610, China
Tel: 862037203765
E-mail: [email protected]

Received Date: May 23, 2016; Accepted Date: June 10, 2016; Published Date: June 15, 2016

Citation: Sheng ZY, Si HJ, Ming LX, Rong CJ, Yi CZ, et al. (2016) The Study of Analytical Identification on Main Monomer Compounds of Spoiled Grass Carp by High Performance Liquid Chromatography of Quadrupole Time of Flight Mass Spectrometry. J Food Process Technol 7:600. doi:10.4172/2157-7110.1000600

Copyright: © 2016 Sheng ZY, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Background: The change of monomer compounds of materials during preservation is becoming increasingly important in the background of food preservation. It can help to understand underlying causes of quality change of materials in preservation substantially. The objective of this study was to infer and analyze the change of main monomer compounds during spoiled Grass carp (Ctenopharyngodon Idellus), and in favor of understanding the quality change of Grass carp in preservation.
Methods and materials: The spoiled Grass carp was studied as materials by High Performance Liquid Chromatography of Quadrupole Time of Flight Mass Spectrometry (HPLC-Q-TOF-MS). HPLC-Q-TOF-MS has two scan modes, positive one and negative one. Special related substances and molecular components of this characteristic fragment ion were identified by quasimolecular ion peaks and accurate molecular weight of fragment ion from high-resolution mass spectrometry.
Results: 46 kinds of monomer compounds were determined in spoiled Grass carp, which have 3 kinds of non-nitrogenous compounds and 43 nitrogenous compounds. 43 nitrogenous compounds including 6 kinds of amino acids (2 kinds of α-amino acids), 10 kinds of amines, 12 kinds of amide compounds, 2 kinds of nitro compounds, 12 kinds of heterocyclic nitrogenous compounds, and 1 kinds of nitriles compound. Conclusion and suggestion: Structure of monomer compounds in fresh and perishable materials can be inferred and identificated by HPLC-Q-TOF-MS. They can be used to increase efficiency in identification and analysis of chemical component. It will be benefit for identification, evolution, and deduction of active ingredients and new compounds of fresh material in preservation.

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