alexa The Study of Inhibitory Effects of Satureja khuzestanica Essence against Exos Gene of MDR Pseudomonas aeruginosa by RT-PCR Technique
ISSN: 2167-7689

Pharmaceutical Regulatory Affairs: Open Access
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Research Article

The Study of Inhibitory Effects of Satureja khuzestanica Essence against Exos Gene of MDR Pseudomonas aeruginosa by RT-PCR Technique

Davoud Esmaeili1*, Fateme Moradi1, Abbas Ali Imani Foladi1 and Abbas Bahador2

1Applied Microbiology Research Center, and Microbiology Department, Baqiyatallah University of Medical Sciences, Iran

2Departments of Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran

*Corresponding Author:
Davoud Esmaeili
Applied Microbiology Research Center, and Microbiology Department
Baqiyatallah University of Medical Sciences, Iran
Tel: 098 2122289943
Fax: 098-21222899
E-mail: [email protected]

Received date: June 08, 2015; Accepted date: September 10, 2015; Published date: September 14, 2015

Citation: Esmaeili D, Moradi F, Foladi AAI, Bahador A (2015) The Study of Inhibitory Effects of Satureja khuzestanica Essence against Exos Gene of MDR Pseudomonas aeruginosa by RT-PCR Technique. Pharmaceut Reg Affairs 4:151. doi:10.4172/2167-7689.1000151

Copyright: © 2015 Esmaeili D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Pseudomonas aeruginosa is an opportunistic pathogen that can cause severe hospital-acquired infections, especially in immune-compromised hosts. P. aeruginosa is notorious for its resistance to antibiotics. Exoenzyme S is one of the several toxins involved in Spreading of infections in burns. It’s been revealed that deletion of genes encoding the components of exoS, in wild-type P. aeruginosa, confers hyper susceptibility to antiphagocytic agents. Antimicrobial and antifungal properties of some herbal medicines were numerously reported. In this study the effect of Satureja khuzestanica extract, an endemic plant of Iran, on the expression level of exoS gene in P. aeruginosa were investigated. For this purpose, MIC was determined for P. aeruginosa. Then, bacteria were treated with S. khuzistanica extract. ExoS and gyrA genes expression in treated and non-treated bacteria, before and after expose was evaluated using RT-PCR technique. Surprisingly, the expression level of exoS gene was decreased in the presence of S. Khuzestanica. However, the expression of gyrA gene that was used as an internal control was not altered before and after treatment with this herb. Based on the results, S. Khuzestanica could play a, major role in lowering the P. aeruginosa resistance to drugs, by reducing exoS gene expression. According to results of current research we hope in future be used it to the clinic with a wider range as a complementary therapy and also for surgery operation and etc.

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