The Use of Real-Time PCR (qPCR) for the Diagnosis of Bovine Herpes virus 5 in Formalin-Fixed, Paraffin-Embedded Bovine Brain Samples Identified as Bovine Unspecific EncephalitisFrancisco Pedraza-Ordoñez1,5*, Ricardo Seiti Yamatogi2,3, Joao Pessoa Araujo Jr.2,3, Noeme Sousa Rocha3,4 and Antonio Carlos Alessi1
- *Corresponding Author:
- Francisco Pedraza-Ordoñez
Department of Veterinary Pathology
Agricultural and Veterinary Science Faculty
FCAV-UNESP, Jaboticabal, SP, Brazil
E-mail: [email protected]
Received date: July 09, 2013; Accepted date: October 10, 2013; Published date: October 14, 2013
Citation: Pedraza-Ordoñez F, Yamatogi RS, Araujo Jr JP, Rocha NS, Alessi AC (2013) The Use of Real-Time PCR (qPCR) for the Diagnosis of Bovine Herpes virus 5 in Formalin-Fixed, Paraffin-Embedded Bovine Brain Samples Identified as Bovine Unspecific Encephalitis. J Veterinar Sci Technol 4:145. doi:10.4172/2157-7579.1000145
Copyright: © 2013 Pedraza-Ordoñez F, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Bovine herpes virus 5 (BoHV-5) is a neurovirulent alpha herpes virus that causes fatal meningoencephalitis and huge economic losses in the livestock and associated industries worldwide; its diagnosis in the laboratory is essential for disease control, immunologic surveillance and effective monitoring programs in endemic areas.
Materials and Methods: In this study, brain samples from 21 Colombian cattle that died of neurological disease, were negative for rabies and were diagnosed with nonspecific encephalitis were processed by real-time PCR (qPCR) in the laboratory of virology at the Institute of Biosciences, Sao Paulo State University, Brazil, to detect BoHV-5 genetic material in tissue preserved in paraffin blocks. Tissues were considered positive for BoHV-5 if ampliï¬Âcation occurred at or before 30 PCR cycles, inconclusive if amplification occurred between 30 and 40 cycles and negative otherwise.
Results and Conclusion: Two cases were positive, demonstrating that qPCR may be useful for confirming herpes encephalitis in acid formalin-fixed tissues with an inconclusive diagnosis.