alexa The use of the Cytogenetic to Identify Mechanisms of Action of an Azo Dye in Allium Cepa Meristematic Cells
ISSN: 2161-0525

Journal of Environmental & Analytical Toxicology
Open Access

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Research Article

The use of the Cytogenetic to Identify Mechanisms of Action of an Azo Dye in Allium Cepa Meristematic Cells

Bruna de Campos Ventura-Camargo, Patrícia Pasquali Parise Maltempi and Maria Aparecida Marin-Morales*
Department of Biology, Institute of Biosciences, São Paulo State University (UNESP), Avenue 24A, 1515, 13506-900, Rio Claro, SP, Brazil
Corresponding Author : Marin-Morales
Department of Biology
Institute of Biosciences, São Paulo State University (UNESP)
Avenue 24A, 1515, 13506-900, Rio Claro, SP, Brazil
Tel: +55 19 3526 4143
Fax: +55 19 3536 0009
E-mail: [email protected]
Received October 28, 2011; Accepted November 26, 2011; Published November 29, 2011
Citation: Ventura-Camargo BC, Maltempi PPP, Marin-Morales MA (2011) The use of the Cytogenetic to Identify Mechanisms of Action of an Azo Dye in Allium Cepa Meristematic Cells. J Environment Analytic Toxicol 1:109. doi: 10.4172/2161-0525.1000109
Copyright: © 2011 Ventura-Camargo BC, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

In the present study, cytotoxic, genotoxic and mutagenic actions of different concentrations (1, 10, 100 and 1000 μg/L) of an azo dye (BDCP – Black Dye Commercial Product) were evaluated using different cytogenetic techniques [(conventional dye, C banding, NOR banding, base-specific fluorochrome banding and fluorescent in situ hybridization (FISH)] applied to the Allium cepa test-organism. The use of conventional cytogenetic staining allowed us to determine that the azo dye induced cell death, chromosomal aberrations, nuclear alteration and micronuclei. By means of chromosome bandings and the FISH technique, it was possible to notice both cell and nucleolar alterations induced by BDCP, whose effects, in a general way, were not specific for any chromosome sites. The abnormalities observed made it possible to infer both aneugenic and clastogenic actions caused by the dye analyzed. The cytotoxic, genotoxic and mutagenic effects of BDCP were not completely eliminated, even after the recovery treatment of the A. cepa roots in water, showing a cumulative potential effect of the referred dye. Due to these results, we could infer that the BDCP may be a dangerous contaminant to the environment and, consequently, to the lives of the organisms exposed to it.

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