alexa The Utilization of the SDS-PAGE Salivary Protein Profil
ISSN: 2157-7579

Journal of Veterinary Science & Technology
Open Access

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Research Article

The Utilization of the SDS-PAGE Salivary Protein Profiles for Clustering Analysis of Bali Cattle (Bos sondaicus/javanicus) and the Taurine or Zebu Cross Breeds in North Lombok District, Indonesia: A Preliminary Study

Masri Junihardy, Maskur M, Soegeng Prasetyo, Muhamad Ali and Sulaiman N Depamede*

Postgraduate Study Program of Management and Livestock Resources, Faculty of Animal Science, Mataram University, Jalan Majapahit 62 Mataram, NTB-83125, Indonesia

*Corresponding Author:
Sulaiman Ngongu Depamede
Postgraduate Study Program of Management and Livestock Resources
Faculty of Animal Science, Mataram University Jalan Majapahit 62 Mataram
NTB-83125, Indonesia
Tel: +62370633603
E-mail: [email protected] (or) [email protected]

Received Date: September 23, 2016; Accepted Date: October 03, 2016; Published Date: October 07, 2016

Citation: Junihardy M, Maskur M, Prasetyo S, Ali M, Depamede SN (2016) The Utilization of the SDS-PAGE Salivary Protein Profiles for Clustering Analysis of Bali Cattle (Bos sondaicus/javanicus) and the Taurine or Zebu Cross Breeds in North Lombok District, Indonesia: A Preliminary Study. J Vet Sci Technol 7:386. doi: 10.4172/2157-7579.1000386

Copyright: © 2016 Junihardy M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

The main aim of this study is to investigate the salivary protein profile of Bali cattle (Bos sondaicus/javanicus) and their crossbred descendents with Bos taurus or Bos indicus in the country side farm of North Lombok District, Indonesia. A total of 52 samples of saliva collected from Bali cattle, a mix of Bali cattle and taurine or zebu cattle, and a pure taurine dairy cattle, were investigated through SDS-PAGE. Intra and inter specific relationships were estimated using Jaccard’s similarity and Euclidean distance index. Dendograms based on cluster analysis, and hierarchical analyses using Ward’s method were developed. It was revealed that cluster analyses of salivary proteins in the range of 17-45 kDa were able to be classified and disengaged from the breed or species in the population in this study. It can thus be concluded that saliva has a good prospect as an alternative biological material for phylogenetic studies in the population. It is explicable that further verification using molecular genetics technology, should be carried out before the concept is decided to be put into daily practice.

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