Therapeutic Potentials of CD151 shRNA in Targeting Metastasis of Triple Negative Breast Cancer Cell Line MDA-MB-231
Gayatri Devi V, Anil Badana, Seema Kumari, Nagaseshu P and Rama Rao Malla*
Department of Biochemistry, Cancer Biology Lab, GIS, GITAM University, Vizag, India
- *Corresponding Author:
- Malla RR
Department of Biochemistry, Cancer Biology Lab, Institute of Science
GITAM University, Visakhapatnam, Andhra Pradesh, 530045, India
E-mail: [email protected], [email protected]
Received Date: February 24, 2016; Accepted Date: April 26, 2016; Published Date: April 28, 2016
Citation: Gayatri Devi V, Badana A, Kumari S, Nagaseshu P, Malla RR (2016) Therapeutic Potentials of CD151 shRNA in Targeting Metastasis of Triple Negative Breast Cancer Cell Line MDA-MB-231. J Cancer Sci Ther 8:104-112. doi:10.4172/1948-5956.1000400
Copyright: © 2016 Gayatri Devi V, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Objective: CD151 is a master regulator of cell adhesion signalling and acts as a promoter in tumour progression. Induction of RNAi through shRNA expression holds great prospective in biomedical research. Bioinformatics approach used to predict potential shRNAs that knockdown CD151. The aim of present study is to investigate the role of CD151 in metastasis of triple negative breast cancer cells using shRNA. Methods: Triple negative breast cancer line, MDA-MB-231 obtained from NCCS Pune, India and expression of CD151 determined using western blot and RT-PCR analysis. Small hairpin RNA (shRNA) was constructed using pSilencer 2.1-U6 puro vector to knockdown CD151 expression. The role of CD151 in proliferation, apoptosis, migration, invasion and cell adhesion was evaluated by silencing CD151 gene using CD151shRNA.
Results: RNA interference technology (RNAi) used to silence CD151 gene expression in MDA-MB-231 cells. Delivery of specific shRNA targeting human endogenous CD151 showed significant growth inhibition of MDA- MB-231 cells. The gene expression study by RT-PCR analysis showed that expression of CD151 at mRNA level reduced six fold with CD151 gene knockdown. CD151 gene silencing for 48h using shRNA decreased proliferation by 62.7%. CD151 knockdown also lead to the significant inhibition of metastasis and induced apoptosis.
Conclusion: CD151 over expression is essential for tumour progression and our study shows that shRNA mediated gene silencing of CD151 decreases the metastasis, thus emphasizing CD151 as a prognostic marker and help in developing new therapeutics for treatment of triple negative breast cancer.