alexa Thermal Fourier Transform Infrared Technique Quickly De
ISSN : 2153-2435

Pharmaceutica Analytica Acta
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Thermal Fourier Transform Infrared Technique Quickly Detects an Early Onset of Glycation-Induced Conformational Changes of Human Serum Albumin

Yu-Ting H1,2, Hui-Fen L2, Shun-Li W3 and Shan-Yang L1*

1Department of Biotechnology and Pharmaceutical Technology, Yuanpei University of Medical Technology, Hsin Chu, Taiwan, ROC

2Department of Biochemical Science and Technology, National Chiayi University, Chiayi, Taiwan, ROC

3Department of Applied Chemistry, National Chiayi University, Chiayi, Taiwan, ROC

*Corresponding Author:
Shan-Yang Lin
Department of Biotechnology and Pharmaceutical Technology
Yuanpei University of Medical Technology, No. 306
Yuanpei street, Hsin Chu 30015, Taiwan, ROC
Tel: +886-3-6102439
Fax: +886-3-6102328
E-mail: [email protected]

Received date: October 27, 2016; Accepted date: November 14, 2016; Published date: November 16, 2016

Citation: Yu-Ting H, Hui-Fen L, Shun-Li W, Shan-Yang L (2016) Thermal Fourier Transform Infrared Technique Quickly Detects an Early Onset of Glycation-Induced Conformational Changes of Human Serum Albumin. Pharm Anal Acta 7:512. doi: 10.4172/2153-2435.1000512

Copyright: © 2016 Yu-Ting H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Numerous potential inhibitors for prevention of advanced glycation end products (AGEs) formation have been extensively investigated for their in vitro and in vivo features, but it would spend a lot of time studying. In the present study, a unique thermal Fourier Transform Infrared (FTIR) combined system as an accelerated method was attempted to simultaneously determine the thermal-dependent conformational changes of human serum albumin (HSA) in the HSA-ribose mixture and examine the onset of the structural transformation from α-helix to β-sheet structures with or without AGEs inhibitors used. The present results clearly indicate that native HSA had an onset temperature at 96°C for the irreversible thermal-induced structural transition from α-helices to β-sheets, whereas HSA-ribose mixture exhibited its onset temperature near at 78°C due to the early occurrence of glycation. However, the onset temperature of the α-helix to β-sheet transition was gradually changed from 78°C to 96°C by increasing the amount of sodium diclofenac or inositol, which was closed to that of the onset temperature of native HSA. This implies that the thermal-induced transition from α-helix to β-sheet for HSA in the HSA-ribose mixture was effectively prevented after adding sodium or inositol. The present study also suggests that this thermal FTIR technique not only rapidly accelerates the conformational changes of HSA-ribose mixture but also directly detects onset temperature of the α-helix to β-sheet transition in real time. This unique thermal FTIR combined system could be a useful tool to screen and evaluate quickly the glycation-induced conformational changes of proteins in a one-step process.


Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version