Thin-Layer Chromatographic Analysis of Boom Flower (Nitrobenzene) in Biological Materials
A sensitive and selective thin layer chromatographic method for the detection and identification of nitrobenzene using furfural as a spray reagent is described. The reduction product of nitrobenzene gives aniline. Which react with furfural reagent to give a red color spot. The reagent does not react with other organo phosphorous (except ethyl parathion), organochloro, carbamate and synthetic pyrethriod insecticides. The reagent is specific and selective only for nitrobenzene. The constituents of viscera (amino acids, peptides, proteins etc.) and plant materials do not interfere with the test. The detection limit for nitrobenzene is about 0.5 μg.