alexa Three - (-) Catechin-O-Rhamnosides from the Eastern Nigeria Mistletoe with Potent Immunostimulatory and Antioxidant Activities
ISSN: 2167-7956

Journal of Biomolecular Research & Therapeutics
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Research Article

Three - (-) Catechin-O-Rhamnosides from the Eastern Nigeria Mistletoe with Potent Immunostimulatory and Antioxidant Activities

Omeje Edwin Ogechukwu1*, Osadebe Patience Ogoamaka1, Akira Kawamura2, Amal Hassan3, Abdessamad Debbab3, Esimone Charles Okechukwu4, Nworu Chukwuemeka Sylvester5, Nwodo Ngozi1 and Procksh Peter3

1Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka. 410001, Enugu, Nigeria

2Department of Chemistry, Hunter College of CUNY, The City University of New York, USA

3Department of Pharmaceutical Biology, Heinrich-Hein University, Bilk, 40225 Düsseldorf, Germany

4Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, PMB 6025, Awka

5Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka. 410001, Enugu, Nigeria

*Corresponding Author:
Omeje Edwin Ogechukwu
Department of Pharmaceutical and Medicinal Chemistry
Faculty of Pharmaceutical Sciences
University of Nigeria, Nsukka. 410001, Enugu, Nigeria
Tel: +234- 803-549-5441
E-mail: [email protected]; [email protected]

Received date: December 19, 2011; Accepted date: February 21, 2012; Published date: February 25, 2012

Citation: Ogechukwu OE, Ogoamaka OP, Kawamura A, Hassan A, Debbab A, et al. (2012) Three - (-) Catechin-O-Rhamnosides from the Eastern Nigeria Mistletoe with Potent Immunostimulatory and Antioxidant Activities. Biomolecul 1:102. doi:10.4172/2167-7956.1000102

Copyright: © 2012 Ogechukwu OE, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

In an attempt to provide further convincing evidence for the variously reported immunomodulatory potentials of mistletoes, bioassay-guided fractionation of the eastern Nigeria mistletoe afforded three compounds: - (-) catechin- 7-O-rhamnoside (1), - (-) catechin-3-O- rhamnoside (2) and a 4′-methoxy-7-O-rhamnoside (3). Their effects on C57BL6 mice splenocytes proliferation and expression of CD69 molecule were determined using flow cytometry techniques and compared to Lipopolysaccharide (LPS; 10 μg/ml) and Concanavalin A (ConA; 2 μg/ml) as standards. The antioxidant study was by the DPPH model with ascorbic acid as standard. The compounds (1-3) at 100 μg/ml showed statistically significant (p<0.05) stimulatory activity with values of 91.49 ± 0.22%, 95.17 ± 0.01% and 94.23 ± 0.07% respectively compared to 2.65 ± 0.33% for the unstimulated control. However, the CD69 expression assay showed only moderate stimulation. Their measured antioxidant potentials (effective concentration; EC50) were high (≤ 55.42 ± 0.99 mg/ml) when compared to ascorbic acid (17.6 ± 1.78 mg/ml). Characterization of the compounds was achieved by chromatographic and spectroscopic methods.

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