TiO2 NANOPARTICLES AS AN NTIBACTERIAL AGENTS AGAINST E. coli
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Microbial resistance represents a challenge for the scientific community to develop new bioactive compounds. In the present study antibacterial effect of TiO2 nanoparticles (<25nm) on Escherichia coli strain was investigated. The MIC50 value for the TiO2nanoprticles was found to be 200 μg/ml for E. coli, which was confirmed by the Colony-forming unit (CFU) of the culture with and without TiO2 nanoparticles inoculated on agar plate. The Inhibition was further confirmed using Disc diffusion assay. It is evident from the zone of inhibition that TiO2 nanoparticles possess potent bactericidal activity. Further, growth curve study of E.coli in the presence and absence of TiO2 nanoparticles has been done, which shows effect of sub-inhibitory and inhibitory concentration of TiO2 nanoparticles against E. coli till 20 hours of incubation. Nanomaterials are known to inactivate cellular enzyme and DNA by binding to electron donating groups such as carboxylates, Amides, Indoles, Hydroxyles, Thiols etc. They cause little pores in bacterial cell walls leading to increased permeability and cell death.