alexa Titanium Dioxide Nanoparticles are not Cytotoxic or Cla
ISSN: 2161-0525

Journal of Environmental & Analytical Toxicology
Open Access

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Research Article

Titanium Dioxide Nanoparticles are not Cytotoxic or Clastogenic in Human Skin Cells

Cynthia L Browning1,2,3, Therry The1,2, Michael D Mason3 and John Pierce Wise Sr.1,2,3*
1Wise Laboratory of Environmental and Genetic Toxicology, University of Southern Maine, Portland ME 04103, USA
2Maine Center for Toxicology and Environmental Health, University of Southern Maine, Portland ME 04103, USA
3Graduate School of Biomedical Science and Engineering, University of Maine, Orono ME 04469, USA
Corresponding Author : John Pierce Wise Sr.
Wise Laboratory of Environmental and Genetic Toxicology
University of Southern Maine
178 Science Building
96 Falmouth Street
Portland ME 04103, USA
Tel: +1 207 228 8050
E-mail: [email protected]
Received July 24, 2014; Accepted August 08, 2014; Published September 06, 2014
Citation: Browning CL, The T, Mason MD, Wise Sr. JP (2014) Titanium Dioxide Nanoparticles are not Cytotoxic or Clastogenic in Human Skin Cells. J Environ Anal Toxicol 4:239 doi: 10.4172/2161-0525.1000239
Copyright: © 2014 Browning CL, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


The application of nanoparticle technology is rapidly expanding. The reduced dimensionality of nanoparticles can give rise to changes in chemical and physical properties, often resulting in altered toxicity. People are exposed dermally to titanium dioxide (TiO2) nanoparticles in industrial and residential settings. The general public is increasingly exposed to these nanoparticles as their use in cosmetics, sunscreens and lotions expands. The toxicity of TiO2 nanoparticles towards human skin cells is unclear and understudied. We used a human skin fibroblast cell line to investigate the cytotoxicity and clastogenicity of TiO2 nanoparticles after 24 h exposure. In a clonogenic survival assay, treatments of 10, 50 and 100 μg/cm2 induced 97.8, 88.8 and 84.7% relative survival, respectively. Clastogenicity was assessed using a chromosomal aberration assay in order to determine whether TiO2 nanoparticles induced serious forms of DNA damage such as chromatid breaks, isochromatid lesions or chromatid exchanges. Treatments of 0, 10, 50 and 100 μg/cm2 induced 3.3, 3.0, 3.0 and 2.7% metaphases with damage, respectively. No isochromatid lesions or chromatid exchanges were detected. These data show that TiO2 nanoparticles are not cytotoxic or clastogenic to human skin cells.


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