alexa T-Lymphocyte Deficiency Exacerbates Behavioral Deficits in the 6-OHDA Unilateral Lesion Rat Model for Parkinson?s Disease | OMICS International | Abstract
ISSN: 2155-9562

Journal of Neurology & Neurophysiology
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Research Article

T-Lymphocyte Deficiency Exacerbates Behavioral Deficits in the 6-OHDA Unilateral Lesion Rat Model for Parkinson?s Disease

Christopher J Wheeler1, Akop Seksenyan1, Yosef Koronyo1, Altan Rentsendorj1, Danielle Sarayba1, Henry Wu1, Ashley Gragg1, Emily Siegel1, Deborah Thomas1, Andres Espinosa1, Kerry Thompson2, Keith Black1, Maya Koronyo-Hamaoui1, Robert Pechnick3 and Dwain K Irvin1*

1Department of Neurosurgery, Cedars-Sinai Medical Center, Los Angeles, California, 90048, USA, Department of Psychiatry, Cedars-Sinai Medical Center, Los Angeles, California, 90048, USA; Occidental College, Los Angeles, CA 90041, USA

2Department of Biology, Occidental College, Los Angeles, CA 90041, USA

3Department of Basic Medical Sciences, College of Osteopathic Medicine of the Pacific Western University of Health Sciences, Pomona, CA 91766, USA

Corresponding Author:
Dwain K. Morris-Irvin
Research Scientist II, Assistant Professor
Department of Neurosurgery, Cedars-Sinai Medical Center
Los Angeles, CA 90048, USA
Tel: 213-550-8293
E-mail: [email protected]

Received date: December 13, 2013; Accepted date: May 30, 2014; Published date: June 05, 2014

Citation: Wheeler CJ, Seksenyan A, Koronyo Y, Rentsendorj A, Sarayba D, et al. (2014) T-Lymphocyte Deficiency Exacerbates Behavioral Deficits in the 6-OHDA Unilateral Lesion Rat Model for Parkinson’s Disease. J Neurol Neurophysiol 5:209. doi:10.4172/2155-9562.1000209

Copyright: © 2014 Wheeler CJ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

T-lymphocytes have been previously implicated in protecting dopaminergic neurons in the substantianigra from
induced cell death. However, the role of T-cells in neurodegenerative models such as Parkinson’s disease (PD) has
not been fully elucidated. To examine the role of T-lymphocytes on motor behavior in the 6-hydroxydopamine (6-
OHDA) unilateral striatal partial lesion PD rat model, we assessed progression of hemi-parkinsonian lesions in the
substantia nigra, induced by 6-OHDA striatal injections, in athymic rats (RNU-/-, T-lymphocyte-deficient) as
compared to RNU-/+ rats (phenotypically normal). Motor skills were determined by the cylinder and D-amphetamine
sulfate-induced rotational behavioral tests. Cylinder behavioral test showed no significant difference between
unilaterally lesioned RNU-/- and RNU-/+ rats. However both unilaterally lesioned RNU-/- and RNU-/+ rats favored
the use of the limb ipsilateral to lesion. Additionally, amphetamine-induced rotational test revealed greater rotational
asymmetry in RNU-/- rats compared to RNU-/+ rats at two- and six-week post-lesion. Quantitative
immunohistochemistry confirmed loss of striatal TH-immunopositive fibers in RNU-/- and RNU-/+ rat , as well as
blood-brain-barrier changes associated with PD that may influence passage of immune cells into the central nervous
system in RNU-/- brains. Specifically, GFAP immunopositive cells were decreased, as were astrocytic end-feet
(AQP4) contacting blood vessels (laminin) in the lesioned relative to contralateral striatum. Flow cytometric analysis
in 6-OHDA lesioned RNU-/+rats revealed increased CD4+ and decreased CD8+ T cells specifically within lesioned
brain. These results suggest that both major T cell subpopulations are significantly and reciprocally altered following
6-OHDA-lesioning, and that global T cell deficiency exacerbates motor behavioral defects in this rat model of PD.

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