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ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
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Research Article

To Identify the Concentration Level of Various Pigments & to Determine Suitable Solvent System for Different Lipstick Samples by Using TLC

Sonal Srivastava, Kapil Verma* and Jyoti Singh Forensic Science, Amity Institute of Forensic Sciences (AIFS), B-Block, Lower Ground Floor, Amity University—Uttar Pradesh, Sec-125, Noida-201303, Uttar Pradesh, India

*Corresponding Author:
Kapil Verma
Amity Institute of Forensic Sciences (AIFS)
B-Block, Lower Ground Floor
Amity University, Uttar Pradesh
Sec-125, Noida-201303, Uttar Pradesh, India
E-mail: [email protected]

Received date: September 02, 2012; Accepted date: October 20, 2012; Published date: October 25, 2012

Citation: Srivastava S, Verma K, Singh J (2012) To Identify the Concentration Level of Various Pigments & to Determine Suitable Solvent System for Different Lipstick Samples by Using TLC. J Chromat Separation Techniq 3:146. doi:10.4172/2157-7064.1000146

Copyright: © 2012 Srivastava S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


30 lipstick samples of different brands of similar color were selected for this study coloring agent was analyzed by thin layer chromatography (TLC) and UV. Using four different solvent systems [Toluene/Benzene (12:8), Toluene/ Acetone (16:4), Toluene/Benzene/Cyclohexane (4:12:4), Toluene/Benzene/Diethyl ether (12:6:2)]. Lipstick samples of colors indistinguishable on visual observation could be grouped into eight subgroups. The work that follows goes on this line of investigation. The study compares the usefulness of two kinds of fluorescent reagents -Yellowescent Fluorescent Latent Prints Powder and Nile Red- for developing latent lip prints, older than one and a half year, on multi colored surfaces. The reagents were used in powder form and luminescence was observed by an alternate light source and ultraviolet light. In principle Nile Red, like lysochromes, have advantage over other Fluorescent powders because it react with fats and physical agents. The main purpose of this study is to identify concentration level of various pigments & to determine which solvent system is best for the particular brand of the lipstick using Thin Layer Chromatography by checking the differences in the respective chromatogram & UV.


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