To Study the Effects of Shenqifuzheng Injection (SFI) on Micro-RNAs in Human Dendritic CellsYuwh H*, Sze WH, Yip DMY, Cho SP, Boost WCS, Zhang MV, Fan Z, Ng K, Ng MCH, Yeung JWC, Hung A, H WH, Chong GSL and Lee RLP
Faculty of Health and Social Sciences, The Hong Kong Polytechnic University, Hong Kong
- *Corresponding Author:
- Yuwh H
Faculty of Health and Social Sciences
The Hong Kong Polytechnic University, Hong Kong
E-mail: [email protected]
Received date: July 24, 2013; Accepted date: January 25, 2014; Published date: January 29, 2014
Citation: Yuwh H, Sze WH, Yip DMY, Cho SP, Boost WCS, et al. (2014) To Study the Effects of Shenqifuzheng Injection (SFI) on Micro-RNAs in Human Dendritic Cells. J Data Mining Genomics Proteomics 5:149. doi: 10.4172/2153-0602.1000149
Copyright: © 2014 Yuwh H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Method: This study used a 2-step approach. By first we were using a robust DC cell line to identify those miRNA that might be involved in DC activation; then followed by confirmation using monocyte-derived DC which was closer to real-life physiological contexts. THP-1 cell, a human monocytic leukemia cell line, has previously been shown to be useful for the in vitro study of DC. In this study, we identified a number of potential miRNA candidates in the DCs activated by SFI using MicroRNA Array of 381 known human miRNAs (Sanger miR Base, version 14). miR-22, miR-107 and miR-200a were then selected from the over 30 potential miRNA candidates with relative expression fold change >2 indentified in accordance to the computational evidences retrieved in the annotation database pipeline miR Base. These three miRNAs were further examined for any change in expression in a confirmatory study using the monocyte-derived DCs (MDDC) from peripheral blood mononuclear cells of healthy normal subjects (n=7) through the quantification by Real Time RT-PCR quantification. These results were correlated with the expression levels of HLA-DR and CD80 on the MDDCs using flow cytometry. Results: The confirmatory results did not show any consistent up-regulation in the expressions of miR22, miR107 and miR-200a due to SFI across the seven MDDC samples. In addition, test results on MDDCs upon treatment of SFI in two different dilutions of 1/20 and 1/40 respectively for 24 hours incubation also demonstrated insignificant expression changes of HLA-DR and CD80 (p>0.05). Furthermore, the correlation of the expression three mi-RNA and the surface expression levels of HLA-DR and CD80 were not statistically significant for MDDCs with treatment of SFI diluted in 1/20 for 1 hour, SFI diluted in 1/20 for 4 hours, and SFI diluted in 1/40 for 1 hour (p>0.05). Conclusions: Increased expression of miRNA of miR-22, miR-107 and miR-200a were found only in the cell line of THP-1, but failed to be shown consistently in the MDDCs from seven human peripheral blood samples. Thus whether these three mi-RNAs were really important epigenetic regulators during the process of SFI on DC remain unknown; and will require more future investigations by using multiple authentic DC sources.