alexa Toward the Quantification of Confrontation (Dual Cultur
ISSN: 2471-2728

Journal of Fertilizers & Pesticides
Open Access

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Research Article

Toward the Quantification of Confrontation (Dual Culture) Test: A Case Study on the Biological control of Pythium aphanidermatum with Trichoderma asperelloides

Babak S Pakdaman1*, Ebrahim Mohammadi Goltapeh2*, Bahram Mohammad Soltani3, Ali Asghar Talebi4, Mohsen Nadepoor2, Joanna S Kruszewska5, Sebastian Pilsyk5, Sabrina Sarrocco6 and Giovanni Vannacci6

1Department of Phytomedicine, Ramin Agricultural and Natural Resources University, Ahwaz, Iran

2Department of Plant Pathology, Agricultural Faculty, Tarbiat Modares University, Tehran, Iran

3Department of Plant Pathology, Agricultural Faculty, Tarbiat Modares University, Tehran, Iran

4Department of Agricultural Entomology, Agricultural Faculty, Tarbiat Modares University, Tehran, Iran

5Laboratory of Fungal Glycobiology, Division of Genetics, Institute of Biochemistry and Biophysics, Warsaw, Poland

6Department of Tree Science, Entomology and Plant Pathology “Giovanni Scaramuzzi”, Faculty of Agriculture, University of Pisa, Pisa, Italy

*Corresponding Author:
Babak Pakdaman
Department of Phytomedicine
Ramin Agricultural and Natural Resources University
Ahwaz, Iran
Tel: +0098 914 8071770
E-mail: [email protected]
Ebrahim Mohammadi Goltapeh
Department of Plant Pathology
Agricultural Faculty
Tarbiat Modares University, Tehran, Iran
E-mail: [email protected]

Received date: June 05, 2013; Accepted date: October 25, 2013; Published date: October 31, 2013

Citation: Pakdaman BS, Goltapeh EM, Soltani BM, Talebi AA, Nadepoor, et al. (2013) Toward the Quantification of Confrontation (Dual Culture) Test: A Case Study on the Biological control of Pythium aphanidermatum with Trichoderma asperelloides. J Biofertil Biopestici 4:137. doi:10.4172/2155-6202.1000137

Copyright: © 2013 Pakdaman BS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Trichoderma species are well-appreciated filamentous fungi applied in agriculture for biological control and biofertilization purposes. One of the primary steps to screen for potent biological control isolates and/to study the effect of gene(s) on the biological control of phytopathogenic fungi and oomycetes is to perform confrontation or dual culture tests. Despite the comprehensiveness of the test, it still suffers from the lack of a reliable methodology for the mathematical data collection and statistical analyses. Here, different aspects of data collection are critically studied and new parameters are introduced. With this method the statistical comparison of different fungal biological control isolates and their counterpart phytopathogenic fungi as well as oomycetes becomes feasible. In the mean time, with this new approach, it becomes possible to statistically analyze the effect of different factors included in the medium of interaction. And with those interested in genetic studies on this type of interactions, the results of this study indicate the probable feasibility of statistical analysis of the possible impact of genetic manipulation and transformation of fungal biological control agents on the biological control of phytopathogenic fungi and oomycetes, and vice versa.


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