alexa Toward the Quantification of Confrontation (Dual Cultur
ISSN: 2471-2728

Journal of Fertilizers & Pesticides
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Toward the Quantification of Confrontation (Dual Culture) Test: A Case Study on the Biological control of Pythium aphanidermatum with Trichoderma asperelloides

Babak S Pakdaman1*, Ebrahim Mohammadi Goltapeh2*, Bahram Mohammad Soltani3, Ali Asghar Talebi4, Mohsen Nadepoor2, Joanna S Kruszewska5, Sebastian Pilsyk5, Sabrina Sarrocco6 and Giovanni Vannacci6

1Department of Phytomedicine, Ramin Agricultural and Natural Resources University, Ahwaz, Iran

2Department of Plant Pathology, Agricultural Faculty, Tarbiat Modares University, Tehran, Iran

3Department of Plant Pathology, Agricultural Faculty, Tarbiat Modares University, Tehran, Iran

4Department of Agricultural Entomology, Agricultural Faculty, Tarbiat Modares University, Tehran, Iran

5Laboratory of Fungal Glycobiology, Division of Genetics, Institute of Biochemistry and Biophysics, Warsaw, Poland

6Department of Tree Science, Entomology and Plant Pathology “Giovanni Scaramuzzi”, Faculty of Agriculture, University of Pisa, Pisa, Italy

*Corresponding Author:
Babak Pakdaman
Department of Phytomedicine
Ramin Agricultural and Natural Resources University
Ahwaz, Iran
Tel: +0098 914 8071770
E-mail: [email protected]
 
Ebrahim Mohammadi Goltapeh
Department of Plant Pathology
Agricultural Faculty
Tarbiat Modares University, Tehran, Iran
E-mail: [email protected]

Received date: June 05, 2013; Accepted date: October 25, 2013; Published date: October 31, 2013

Citation: Pakdaman BS, Goltapeh EM, Soltani BM, Talebi AA, Nadepoor, et al. (2013) Toward the Quantification of Confrontation (Dual Culture) Test: A Case Study on the Biological control of Pythium aphanidermatum with Trichoderma asperelloides. J Biofertil Biopestici 4:137. doi:10.4172/2155-6202.1000137

Copyright: © 2013 Pakdaman BS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Trichoderma species are well-appreciated filamentous fungi applied in agriculture for biological control and biofertilization purposes. One of the primary steps to screen for potent biological control isolates and/to study the effect of gene(s) on the biological control of phytopathogenic fungi and oomycetes is to perform confrontation or dual culture tests. Despite the comprehensiveness of the test, it still suffers from the lack of a reliable methodology for the mathematical data collection and statistical analyses. Here, different aspects of data collection are critically studied and new parameters are introduced. With this method the statistical comparison of different fungal biological control isolates and their counterpart phytopathogenic fungi as well as oomycetes becomes feasible. In the mean time, with this new approach, it becomes possible to statistically analyze the effect of different factors included in the medium of interaction. And with those interested in genetic studies on this type of interactions, the results of this study indicate the probable feasibility of statistical analysis of the possible impact of genetic manipulation and transformation of fungal biological control agents on the biological control of phytopathogenic fungi and oomycetes, and vice versa.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords