Giridhara Basappa, Vadlapudi Kumar, Sarojini BK, Poornima DV, Hari Gajula, Torankumar Sannabommaji and Rajashekar J
Anisomeles indica (L.) Kuntze belongs to Lamiaceae, is used against inflammation and gastric dysfunctions. Tilldate there are no reports on toxicological evaluation of A. indica leaf flavonoids. In the present study, in vivo and in vitro toxicity evaluation of A. indica leaf flavonoid fraction was carried out to assess its safety. Acute toxicity results in mice suggest that, LFF is non-toxic, as the treated animals survived beyond 14-day observation period and with no mortality and toxic signs or symptoms. Subacute oral toxicity study was carried out for 28 days in Swiss albino mice both sexes. Data revealed no statistically significant dose-related effects on food consumption, body weight gain, clinical signs in LFF treated animals. Urine analysis, hematological parameters, serum biochemical examination and histopathology revealed no adverse effects even at 1000 mg/kg body weight/day. In vitro chromosome aberration assay in human lymphocytes revealed no clastogenic effect at 24 h and 48 h LFF treatments. Cytotoxicity results against human cancer cell lines KB, HepG2, HT-29 and HEK-293 suggests, LFF is cytotoxic to four human cancer cell lines with IC50 values 36.57, 40.0, 48.6, 68.58 μg/mL respectively. In conclusion A. indica LFF appears to be safe for oral administration in humans, may be used in traditional medicine.
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