alexa Transcriptomic Profiling of Mice Primary Cortical Neurons in Response to Medium Change
ISSN: 2329-8936

Transcriptomics: Open Access
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Transcriptomic Profiling of Mice Primary Cortical Neurons in Response to Medium Change

Juan Andrés Rubiolo1, Carmen Vale1, Victor Martín1, Aida G Méndez1, Andrea Boente-Juncal1, Mercedes R Vieytes2 and Luis Botana1*

1Department of Pharmacology, Faculty of Veterinary Medicine, University of Santiago de Compostela (USC), Lugo Campus, Lugo, Spain

2Department of Physiology, Faculty of Veterinary Medicine, University of Santiago de Compostela (USC), Lugo Campus, Lugo, Spain

*Corresponding Author:
Prof. Luis M Botana
Department of Pharmacology
Faculty of Veterinary Medicine
University of Santiago de Compostela (USC)
Campus Lugo, 27002 Lugo, Spain
Tel: 0034982822223
Fax: 0034982822223
E-mail: [email protected]

Received date: December 19, 2016; Accepted date: December 30, 2016; Published date: December 31, 2016

Citation: Rubiolo JA, Vale C, Martín V, Méndez AG, Boente-Juncal A, et al. (2016) Transcriptomic Profiling of Mice Primary Cortical Neurons in Response to Medium Change. Transcriptomics 4:138. doi:10.4172/2329-8936.1000138

Copyright: © 2016 Rubiolo JA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The first in vitro system for the culture of primary neurons was developed in 1977. Since then, the culture systems for hippocampal and cortical neurons, as well as neuron cell lines, have improved dramatically. Primary neuronal cultures provide a defined environment where neurons can be studied in isolation from other cell types and are a powerful tool for studying cellular and molecular mechanisms of neuronal development, aging, and death. However, the changes that occur in neurons during culture in relation to cell differentiation and medium composition have not been thoroughly characterized. To study the effect of medium change in primary neurons in culture, we performed a transcriptomic analysis using whole mouse genome microarrays, for neurons between 7 and 10 days in vitro after medium change. We show that even though neurons in culture present an expression profile pointing to differentiation with time in culture, medium change induces a transient and partial de-differentiation.

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords