Tributylamine Facilitated Separations of Fucosylated Chondroitin Sulfate (Fucs) by High Performance Liquid Chromatography (HPLC) into its Component Using 1-Phenyl-3-Methyl- 5-Pyrazolone (PMP) Derivatization
- *Corresponding Author:
- Siddiquee S
Biotechnology Research Institute
University Malaysia Sabah
Jln UMS, 88400 Kota Kinabalu
Tel: 60 88320000 ext. 8467
Fax: +60 88 320993
E-mail: [email protected] my, [email protected]
Received date: November 10, 2014; Accepted date: December 07, 2014; Published date: December 10, 2014
Citation: Myron P, Siddiquee S, Azad SA, Yong YS. (2015) Tributylamine Facilitated Separations of Fucosylated Chondroitin Sulfate (Fucs) by High Performance Liquid Chromatography (HPLC) into its Component Using 1-Phenyl- 3-Methyl-5-Pyrazolone (PMP) Derivatization. J Chromatogr Sep Tech 6:256. doi: 10.4172/2157-7064.1000256
Copyright: © 2015 Myron P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Monosaccharide characterization study can give valuable information on newly discovered intact mucopolysaccharide. The compound fucosylated chondroitin sulfate (FuCS) is a unique mucopolysaccharide having various bioactive properties reported only in targeted holothurians species. The monosaccharide composition of FuCS from a sea cucumber, Holothuria arenicola was studied by comparing a modified and conventional 1-phenyl- 3-methyl-5-pyrazolone (PMP) derivatization method where tributylamine addition was found to improve the chromatographic conditions. The monosaccharide proportion of FuCS from H. arenicola was determined to be 1:1:0.44 for glucuronic acid: N-acetylgalactosamine: fucose, respectively. Thus, the FuCS monosaccharide ratio of H. arenicola was first reported and this method is more useful for structurally similar compound analysis.