alexa Trichoderma atroviride 102C1 Mutant: A High Endoxylanase Producer for Assisting Lignocellulosic Material Degradation | OMICS International | Abstract
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
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Research Article

Trichoderma atroviride 102C1 Mutant: A High Endoxylanase Producer for Assisting Lignocellulosic Material Degradation

Mariana Menezes Quadros de Oliveira1, André Luiz Grigorevski Grigorevski-Lima1, Marcella Novaes Franco-Cirigliano1, Rodrigo Pires do Nascimento2*, Elba Pinto da Silva Bon3 and Rosalie Reed Rodrigues Coelho1

1Federal University of Rio de Janeiro (UFRJ), Center for Health Sciences (CCS), Institute of Microbiology Professor. Paulo Goes, Department of Microbiology Overall, Avenida Carlos Chagas Filho, 373, Block I, Lab 055, CEP: 21941-902. Rio de Janeiro, RJ, Brazil

2Federal University of Rio de Janeiro (UFRJ), the Technology Center (TC), School of Chemistry, Department of Biochemical Engineering, at Avenida Athos da Silveira Ramos, 149, Block E, Room 203, CEP: 21941-909 Rio de Janeiro, RJ, Brazil

3Federal University of Rio de Janeiro (UFRJ), Centre for Mathematical Sciences and Nature (CCMN), Institute of Chemistry, Department of Biochemistry, Avenue Athos da Silveira Ramos 149, Block A, Room 539, CEP: 21941-909 Rio de Janeiro, RJ, Brazil

*Corresponding Author:
Rodrigo Pires do Nascimento
School of Chemistry
Technology Center. Federal University of Rio de Janeiro. Athos avenue of Silveira Ramos 149
Block E, Room 203. Ciudad Universitaria. Fundão Island. CEP: 21941-909
Rio de Janeiro, RJ, Brazil
Tel: + 55213938 8863
E-mail:[email protected]

Published date: April 08, 2014; Published date: May 22, 2014; Published date: May 29, 2014

Citation: Oliveira MMQ, Grigorevski-Lima AL, Franco-Cirigliano MN, Nascimento RP, Bon EPS, et al. (2014) Trichoderma atroviride 102C1 Mutant: A High Endoxylanase Producer for Assisting Lignocellulosic Material Degradation. J Microb Biochem Technol 6: 236-241. doi:10.4172/1948-5948.1000150

Copyright: © 2014 de Oliveira MMQ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Endoxylanases have played an important role in many industrial processes as bleachers to kraft pulp, animal feeds and baked goods. Also, nowadays, a special attention has been devoted to the role of these enzymes in saccharification of lignocellulose biomass for biofuels production. Trichoderma species are among fungi those that have been most extensively studied, due to their efficient production of these enzymes. Among the different strategies for improving the production and biochemical aspects of enzymes of commercial interest, mutations induced using chemical agents and/or physical devices can be cited. In the present strain T. atroviride 102C1 was obtained by using UV light and nitrosoguanidine as mutagenic agents. A factorial design (central composite rotational design, CCRD) was performed to estimate the optimal levels of C (sugarcane bagasse) and N (corn steep liquor) sources for best xylanase production. After the CCRD, the 102C1 mutant strain showed increased activity of 340% for xylanase production when compared to the wild type. The enzyme was partially characterized according to its pH and temperature profile, also using CCRD. The characterization of 102C1 mutant strain as a high endoxylanase producer allows its use in biotechnological applications, particularly in the hydrolysis of lignocellulosic biomass for biorefinary proposes.

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