Tumor Specific Oligomeric Forms of Nucleophosmin
- *Corresponding Author:
- Dr. Natalia Vladimirova
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences
ul. Miklukho-Maklaya 16/10
117997 Moscow, Russia
E-mail: [email protected]
Received Date: September 30, 2011; Accepted Date: October 16, 2011; Published Date: October 18, 2011
Citation: Vladimirova NM, Pisareva MA, Zharskaya OO, Deineko NL, Bulycheva TI, et al. (2011) Tumor Specific Oligomeric Forms of Nucleophosmin. J Cancer Sci Ther 3: 205-212. doi:10.4172/1948-5956.1000090
Copyright: © 2011 Vladimirova NM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: According to recent data malignization of cells is accompanied not only by overexpression of protein B23/nucleophosmin, but also by formation of its new structural forms including unusual oligomers. The aim of this study was to evaluate the structural features of nucleophosmin in tumor cells. Materials and methods: Structural state of nucleophosmin was analyzed in different human tumor cells (HeLa, NGP, Hep G2, Osa-CL, Jurkat, Ramos, K-562) and human lymphocytes stimulated to proliferate by phytohemagglutinin. Commercially available monoclonal antibodies and new obtained by us antipeptide antibodies were used for analysis of monomer-oligomer state of nucleophosmin by immunochemical method and for determination of intracellular localization of monomers and oligomers by immunocytochemical method. Results: We revealed unusual SDS-resistant oligomeric forms of nucleophosmin in tumor cells of different type. Using protein chemistry strategy we showed the presence of truncated B23 isoforms in HeLa cells and their ability to form SDS-resistant oligomers. For the first time we created antipeptide antibodies which allowed differentiate monomeric and oligomeric nucleophosmin forms in tumor cells. Using these antibodies we showed different intracellular localization of monomers and oligomers in tumor cells. Conclusions: We propose that formation of SDS-resistant oligomeric forms of nucleophosmin is a common feature of human tumor cells and their detection with described antipeptide antibodies may be used for tumor diagnostics.