Two Dimensional Protein Map Standardization of Human Bone Marrow Stromal Cells
- *Corresponding Author:
- Ruszymah Idrus
Tissue Engineering Centre
Universiti Kebangsaan Malaysia Medical Centre
Universiti Kebangsaan Malaysia
E-mail: [email protected], [email protected]
Received Date: February 27, 2013; Accepted Date: April 25, 2013; Published Date: April 29, 2013
Citation: Ng MH, Doustjalali SR, Pathak R, Sabet NS, Khor SF, et al. (2013) Two Dimensional Protein Map Standardization of Human Bone Marrow Stromal Cells. J Proteomics Bioinform 6: 085-087. doi: 10.4172/jpb.1000266
Copyright: © 2013 Ng MH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Human bone marrow stromal cells (BMSCs) have been hailed as a promising source for cell therapy and tissue regeneration due to its multipotent nature. Bone marrow stromal cells differentiate into various tissues at the cues from its surrounding. Proteomics is a powerful tool to elucidate cellular mechanisms. The present study established a reproducible protocol to display the complete qualitative 2-D protein map of BMSCs. Results showed the standard optimization of the 2-D protein mapping at pH 3-10 with 60 μg protein loading in freshly isolated mononuclear cells, cultured BMSCs (P0) and BMSCs (P2). This protocol could be further used, along with identification of differentially expressed protein by mass spectrometry, for thorough understanding of the molecular events involved, which would enable a better control of the various differentiation processes of BMSCs.