alexa Two Dimensional Protein Map Standardization of Human Bone Marrow Stromal Cells
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
Open Access

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Research Article

Two Dimensional Protein Map Standardization of Human Bone Marrow Stromal Cells

Min H Ng1,5, Saeid R Doustjalali4,5, Rajiv Pathak6, Negar S Sabet7, Sok F Khor1, Yogeswaran Lokanathan1, Ali N Ghani3, Aminuddin Saim2, Marzalina Mansor4 and Ruszymah Idrus1,5*

1Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre, Universiti Kebangsaan, Malaysia

2Ear, Nose and Throat Consultant Clinic, Ampang Puteri Specialist Hospital, Kuala Lumpur, Malaysia

3Department of Orthopaedics and Traumatology, Ampang Puteri Specialist Hospital, Kuala Lumpur, Malaysia

4Biotechnology Unit, Forest Research Institute, Malaysia

5Department of Physiology, Faculty of Medicine Universiti Kebangsaan, Malaysia

6Faculty of Medicine, Kota Damansara Campus, SEGi University, Selangor, Malaysia

7Tropical Infectious Disease Research and Education Center (TIDREC), Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia

*Corresponding Author:
Ruszymah Idrus
Tissue Engineering Centre
Universiti Kebangsaan Malaysia Medical Centre
Universiti Kebangsaan Malaysia
Fax: +603-26939687
E-mail: [email protected], [email protected]

Received Date: February 27, 2013; Accepted Date: April 25, 2013; Published Date: April 29, 2013

Citation: Ng MH, Doustjalali SR, Pathak R, Sabet NS, Khor SF, et al. (2013) Two Dimensional Protein Map Standardization of Human Bone Marrow Stromal Cells. J Proteomics Bioinform 6: 085-087. doi: 10.4172/jpb.1000266

Copyright: © 2013 Ng MH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Human bone marrow stromal cells (BMSCs) have been hailed as a promising source for cell therapy and tissue regeneration due to its multipotent nature. Bone marrow stromal cells differentiate into various tissues at the cues from its surrounding. Proteomics is a powerful tool to elucidate cellular mechanisms. The present study established a reproducible protocol to display the complete qualitative 2-D protein map of BMSCs. Results showed the standard optimization of the 2-D protein mapping at pH 3-10 with 60 μg protein loading in freshly isolated mononuclear cells, cultured BMSCs (P0) and BMSCs (P2). This protocol could be further used, along with identification of differentially expressed protein by mass spectrometry, for thorough understanding of the molecular events involved, which would enable a better control of the various differentiation processes of BMSCs.

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