Ultrasonication Extraction - HPLC-VIS Fluorescence Method Validation for Trace Analysis of Oil and Grease in Water
Mohammed Khaled Elbadawy Babikir*
Petro Energy Oil Exploration and Production Company, Sudan
- *Corresponding Author:
- Mohammed Khaled Elbadawy Babikir
Petro Energy Oil Exploration
and Production Company (Petro Energy), Sudan
E-mail: [email protected]
Received Date: March 30, 2017 Accepted Date: April 13, 2017 Published Date: April 16, 2017
Citation: Babikir MKE (2017) Ultrasonication Extraction - HPLC-VIS Fluorescence Method Validation for Trace Analysis of Oil and Grease in Water. J Environ Anal Chem 4: 193. doi:10.41722380-2391.1000193
Copyright: © 2017 Babikir MKE. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A new method was developed to determine the oil and grease in water by HPLC-Vis fluorescence technique. Ultrasonication liquid-liquid extraction method was applied by using 1 ml of toluene to 100 ml of water. The analysis was carried out on a LC-10AT-VP chromatograph with a fluorescence RF. 10 a syknm detector equipped with a varian CN (250 × 4.6 μm) Column. The separation was preformed isocratically using a mixture of 90% solvent (n-Hexane) with 10% solvent (Toluene). Asphaltenic compounds were established to be the main fluorescence chromophores (excitation wave length at 420 nm and emission wave length at 500 nm). The correlation coefficient was (0.992), recovery ranged from 92% to 108% and the limit of detection was found to be 0.06 μg.L-1. The method can be utilized successfully for determination of oil and grease in water samples.