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Updated Review on Micellar Electro kinetic Chromatography | OMICS International | Abstract
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
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Research Article

Updated Review on Micellar Electro kinetic Chromatography

Alagar Raja M1*, Bhargav KS1, Banji D1, Selva Kumar D2

1Department of Pharmaceutical Analysis & Quality Assurance, Nalanda College of Pharmacy, Nalgonda, India

2School of Pharmacy, Taylors University, Subangjaya, Malaysia

*Corresponding Author:
Alagar Raja M
HOD, Department of Pharmaceutical Analysis & Quality Assurance
Nalanda College of Pharmacy, India
E-mail: [email protected]

Received date: April 17, 2014; Accepted date: June 26, 2014; Published date: July 17, 2014

Citation: Alagar RM, Bhargav KS, Banji D, Selva Kumar D (2014) Updated Review on Micellar Electro kinetic Chromatography. J Chromatograph Separat Techniq 5:231. doi:10.4172/2157-7064.1000231

Copyright: © 2014 Alagar Raja M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


In this review article the basic principle of separation process during Micellar electro kinetic chromatography (MEKC) are described. The separation mechanism in MEKC is based on differences in equilibrium between an aqueous Phase and micellar Phase. These Phases are moving different velocities, due to a combination of electrophoresis & electro osmosis. It is a useful branch of capillary electrophoresis (CE) that utilizes surfactant above critical micelle concentration (CMC) as pseudo-stationary phase. MEKC can be employed to separate both charged and neutral molecules, individually or simultaneously, including chiral compounds. MEKC benefits from high peak efficiency due to electro-osmotic flow (EOF) in the separation capillary, compounded with large variety of synthetic surfactants, organic modifiers, temperature and variable separation voltage has made MEKC the method of choice for separation scientists. In this review, we present the introduction of CE, fundamentals of surfactant chemistry as it relates to MEKC, separation principles in MEKC including equations involved in calculating separation parameters (capacity factor, resolution etc.).


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