alexa Updating: Cryptococcosis Diagnostic Aspects | OMICS International | Abstract
ISSN 2155-6113

Journal of AIDS & Clinical Research
Open Access

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Review Article

Updating: Cryptococcosis Diagnostic Aspects

Filipe A Colombo1, Rogério E Brasiliense2, Alexandre Conde2, Daniel Galafassi2 and Alexandra F Gazzoni1*

1Oral Microbiology and Pathology Testing Service Laboratory, School of Dentistry, Faculdade da Serra Gaúcha, 95020-472, Caxias do Sul, Brazil

2School of Dentistry, Faculdade da Serra Gaúcha, 95020-472, Caxias do Sul, Brazil

*Corresponding Author:
Alexandra F. Gazzoni
Oral Microbiology and Pathology Testing Service Laboratory
School of Dentistry, Faculdade da Serra Gaúcha
Caxias do Sul, 95020-472, Brazil
Tel: 555421016006
E-mail: [email protected]

Received date: September 19, 2014; Accepted date: November 24, 2014; Published date: November 28, 2014

Citation: Gazzoni FA, Brasiliense RE, Conde A, Galafassi D, Gazzoni AF (2014) Updating: Cryptococcosis Diagnostic Aspects. J AIDS Clin Res 5:391. doi:10.4172/2155-6113.1000391

Copyright: © 2014 Colombo FA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Currently, AIDS represents the most common risk factor for the development of cryptococcosis. Despite of rapid scale-up of antiretroviral therapy programs and global investement in HIV care over the past decade, the decline in incidende and mortality rates associated with cryptococcosis has not been commensurate. This article puts into context recent diagnostics studies and morphologies - with special attention to laboratory diagnosis based on classical mycology: (a) histopathology through the histochemical techniques of hematoxilin and eosin, periodic acid-Schiff, and Grocott’s silver, as well special histochemical techniques such as Mayer’s mucicarmine and Fontana-Masson; (b) cultures by Sabouraud’s medium, brain-heart infusion agar, canavanineglycine- bromothymol blue agar, in addition to niger seed agar medium and lysis-centrifugation blood-culturing technique; (c) serology by cryptococcal antigen test and cryptococcal antigen lateral flow assay. In this paper, we also discussed the new rapid point-of-care lateral flow assay to be used to screening HIV-infected person with CD4 counts of less than 100 cells /μL. It is noteworthy that laboratory findings should be interpreted considering the clinical and epidemiological history of all patients. Consequently, diagnostic tools are only properly used, if the treating physician is aware of the propensity of patients to acquire a fungal infection

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