alexa Use of Agro Waste Biomass for α-Amylase Production by Anoxybacillus amylolyticus: Purification and Properties | OMICS International
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
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Research Article

Use of Agro Waste Biomass for α-Amylase Production by Anoxybacillus amylolyticus: Purification and Properties

Ilaria Finore1, Paola Di Donato1,2, Annarita Poli1, Betul Kirdar3, Ceyda Kasavi3, Ebru O Toksoy4, Barbara Nicolaus1 and Licia Lama1*

1CNR-National Research Council of Italy, Institute of Biomolecular Chemistry, Via Campi Flegrei 34, 80078 Pozzuoli, NA, Italy

2Department of Sciences and Technology, University of Naples "Parthenope" Centro Direzionale Napoli (Isola C4) 80143 Naples, Italy

3Department of Chemical Engineering, Bogazici University, Bebek, 34342 Istanbul, Turkey

4Department of Bioengineering, Marmara University, Goztepe Campus, 34722 Istanbul, Turkey

*Corresponding Author:
Licia Lama
CNR-National Research Council of Italy
Institute of Biomolecular Chemistry
Via Campi Flegrei 34, 80078 Pozzuoli, NA, Italy
Tel: +39 06 49931
E-mail: [email protected]

Published date: June 16, 2014; Published date: July 23, 2014; Published date: July 30, 2014

Citation: Finore I, Donato PD, Poli A, Kirdar B, Kasavi C, et al. (2014) Use of Agro Waste Biomass for a-Amylase Production by Anoxybacillus amylolyticus: Purification and Properties. J Microb Biochem Technol 6:320-326. doi:10.4172/1948-5948.1000162

Copyright: © 2014 Finore I, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Abstract

Knowledge accumulated from fundamental and applied studies on Anoxybacillus suggests that this genus can serve as a good alternative in many applications related to starch and lignocellulosic biomasses, waste treatment, enzyme technology, and bioenergy production. We investigated the purification, biochemical characterization and immobilization of a thermostable α-amylase from the thermophilic Anoxybacillus amylolyticus, strain MR3CT, isolated in Antarctica and its production on vegetable wastes. In particular, the rhizome from Arundo donax, waste biomass of Cynara cardunculus and potato peels were tested either in Submerged Fermentation (SmF) and Solid State Fermentation (SSF) conditions. The amylase from A. amylolyticus, with a molecular weight of about 60 kDa, displayed an optimum enzyme activity at 60°C and pH 5.6. Moreover, by retaining up to 70% of total activity after 48 h at 60°C, it showed high thermostability in the presence of 2 mM calcium ion. The immobilized enzyme maintained the 48% of its initial activity after the sixth reuse. The optimal conditions for its production in SmF were achieved at 60°C for 24 h with 1% of rhizome from Arundo donax, which was about 2126 U/gds. SSF cultures reached maximum α-amylase yield (102 U/gds) when grown on waste biomass of Cynara cardunculus as substrate, with a substrate-water ratio of 1:1 (w/v), and incubation at 60°C for 4 days. In this study, rhizome of A. donax resulted to be a good substrate for amylase production in SmF thus allowing a cheaper alternative to obtain amylolytic enzymes. Indeed by using rhizomes from A. donax as growth substrate it was possible to recovery an amylase activity level higher than that obtained by synthetic complex medium. Amylase production was also investigated under SSF conditions by using the above listed wastes as sole carbon source for A. amylolyticus growth. Under these conditions, C. cardunculus gave a higher enzyme yield per reactor volume.

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