alexa Use of Dot Blots Analysis in the Separation of Anti-HIV Antibodies in Animals | OMICS International | Abstract
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
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Research Article

Use of Dot Blots Analysis in the Separation of Anti-HIV Antibodies in Animals

Angel Alberto Justiz Vaillant1*, Norma McFarlane-Anderson2, Patrick Eberechi Akpaka1, Monica P Smikle3, Niurka Ramirez4
and Armando Cadiz5

1Department of Para-Clinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad & Tobago

2Department of Basic Medical Sciences, The University of the West Indies, Mona campus, Jamaica

3Department of Microbiology, The University Hospital of West Indies, Mona campus, Jamaica

4Department of Internal Medicine, Freyre de Andrade Hospital, Havana city, Cuba

5Carlos J. Finlay Vaccine and Serum Institute, Havana, Cuba

*Corresponding Author:
Angel Alberto Justiz Vaillant
Department of Para-Clinical Sciences
Faculty of Medical Sciences
The University of the West Indies
St. Augustine, Trinidad & Tobago
E-mail: [email protected]

Received Date: April 12, 2013; Accepted Date: April 22, 2013; Published Date: April 26, 2013

Citation: Justiz Vaillant AA, McFarlane-Anderson N, Akpaka PE, Smikle MP, Ramirez N, et al. (2013) Use of Dot Blots Analysis in the Separation of Anti-HIV Antibodies in Animals. J Chromat Separation Techniq 4:181. doi: 10.4172/2157-7064.1000181

Copyright: © 2013 Justiz Vaillant AA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

In this study antibodies against the fragment 254-274 from gp120 of human immunodeficiency virus (HIV) were produced in cats, rats and chicken that can be used as reagents in the preparation of diagnostic kits. Enzyme linked immunosorbent assay showed the presence of anti-HIVgp120 antibodies. Dot blot analysis was used as a separation technique and confirmed the results, proving its efficiency in the detection of proteins. This study also demonstrated that orally given antibodies to an animal as oral vaccine, initiated immune responses of the antibodies in the animals which may be useful for protection, antibody purification and development of reagents for immunodiagnostic procedures.

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