Use of Dot Blots Analysis in the Separation of Anti-HIV Antibodies in Animals
- *Corresponding Author:
- Angel Alberto Justiz Vaillant
Department of Para-Clinical Sciences
Faculty of Medical Sciences
The University of the West Indies
St. Augustine, Trinidad & Tobago
E-mail: [email protected]
Received Date: April 12, 2013; Accepted Date: April 22, 2013; Published Date: April 26, 2013
Citation: Justiz Vaillant AA, McFarlane-Anderson N, Akpaka PE, Smikle MP, Ramirez N, et al. (2013) Use of Dot Blots Analysis in the Separation of Anti-HIV Antibodies in Animals. J Chromat Separation Techniq 4:181. doi: 10.4172/2157-7064.1000181
Copyright: © 2013 Justiz Vaillant AA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In this study antibodies against the fragment 254-274 from gp120 of human immunodeficiency virus (HIV) were produced in cats, rats and chicken that can be used as reagents in the preparation of diagnostic kits. Enzyme linked immunosorbent assay showed the presence of anti-HIVgp120 antibodies. Dot blot analysis was used as a separation technique and confirmed the results, proving its efficiency in the detection of proteins. This study also demonstrated that orally given antibodies to an animal as oral vaccine, initiated immune responses of the antibodies in the animals which may be useful for protection, antibody purification and development of reagents for immunodiagnostic procedures.