alexa Use of Nigella sativa Linn. Supercritical Carbon Dioxid
ISSN: 2167-0412

Medicinal & Aromatic Plants
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Use of Nigella sativa Linn. Supercritical Carbon Dioxide Extract for Targeting the Angiogenesis Cascade

Baharetha HM1, Nassar ZD2, Ahamed MK3, Aisha AFA1, Alfadly SO4, Kadir MOA5 and Majid AMSA1*
1EMAN Testing and Research Laboratory, Department of Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden 11800, Pulau Pinang, Malaysia
2School of Pharmacy, University of Queensland, Woolloongabba, QLD 4102, Australia
3EMAN Biodiscoveries Sdn. Bhd., Suite 126, Level 1, Eureka Complex, Universiti Sains Malaysia (USM) Campus, Minden-11800, Penang, Malaysia
4College of Medicine and Medical Sciences, Hadramout University, Mukalla, Yemen
5Department of Environmental Technology, School of Industrial Technology, Universiti Sains Malaysia, Minden 11800, Pulau Pinang, Malaysia
Corresponding Author : Majid AMSA
EMAN Testing and Research Laboratory
Department of Pharmacology, School of Pharmaceutical Sciences
Universiti Sains Malaysia, Minden 11800, Pulau Pinang, Malaysia
Tel: +60124230842
Fax: +6046534582
E-mail: [email protected]
Received February 23, 2016; Accepted March 03, 2016; Published March 07, 2016
Citation: Baharetha HM, Nassar ZD, Ahamed MK, Aisha AFA, Alfadly SO, et al. (2016) Use of Nigella sativa Linn. Supercritical Carbon Dioxide Extract for Targeting the Angiogenesis Cascade. Med Aromat Plants 5:241. doi:10.4172/2167-0412.1000241
Copyright: © 2016 Baharetha HM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

The aim of this study was to investigate the antiangiogenic effect of the Nigella sativa seeds extracts. Twelve extracts were prepared from the N. sativa seeds by supper critical carbon dioxide by varying extraction pressure and temperature. GC-MS analysis revealed the presence of 22 compounds, mainly thymoquinone (TQ), thymohydroquinone and androstane-3,17-diol. The extracts showed significant antioxidant activity, and cell viability study on human umbilical vein endothelial cells (HUVECs) indicated that two extracts (A3 and B3), prepared at higher temperature (60°C) and the lower pressures (2500 and 3000 psi), were the most potent anti-proliferative extracts; the median inhibitory concentrations were 41.5 ± 1.9 and 60.2 ± 2.2 μg/mL, respectively. The extracts down-regulated the expression of vascular endothelial growth factor (VEGF) in endothelia cells causing inhibition of various events of the angiogenesis cascade including; cell proliferation, endothelial cell migration and differentiation on matrigel and consequently inhibited tube formation. The extracts also showed significant inhibition of angiogenesis in vivo by inhibiting the vascularization of the chicken embryo chorioallantoic membrane (CAM). The antiangiogenic activity observed could be due to the anti-oxidant nature of the extract. This study highlights the importance of N. sativa SCCO 2 oil as anti-angiogenic agent.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords