Use of Vitellogenin as Biomarker Indicator in Sex Identification of Giant Grouper (Epinephelus lanceolatus)Daud Om A*1, Jasmani S2, Sung YY3 and Bolong AA3
- *Corresponding Author:
- Daud Om A
Fisheries Research Institute (FRI) Tanjong Demong
Tel: +60 9-668 4100
E-mail: [email protected]
Received Date: June 15, 2016; Accepted Date: August 05, 2016; Published Date: August 12, 2016
Citation: Daud Om A, Jasmani S, Sung YY, Bolong AA (2016) Use of Vitellogenin as Biomarker Indicator in Sex Identification of Giant Grouper (Epinephelus lanceolatus). Poult Fish Wildl Sci 4:163. doi:10.4172/2375-446X.1000163
Copyright: © 2016 Daud Om A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Vitellogenin, the egg yolk precursor protein, has studied as biomarker for sex identification of giant grouper (Epinephelus lanceolatus). Vitellogenin was synthesized successfully in liver of male giant grouper by implanted with 2 mg/kg of Estradiol-17-ß (E2). The levels of vitellogenin (vtg) in blood of E2-injected fish were comparable with those found in the blood of naturally male and female by observed with SDS-PAGE. With antigen from purified plasma E2-treated was produced polyclonal antibody of giant grouper after immunization process with rabbit for 72 days. Analyst by ELISA has demonstrated polyclonal antibody was developed within 2 weeks after primary immunization and greatest differences in absorbance at 1:8,000 dilutions. Validation by western blot, has verified vitellogenin as positive control (in female natural) and negative control (in male natural). It’s showed that the antigens vtg of giant grouper polyclonal antibody recognized the purified giant grouper vtg in natural female and treated estradiol male. The information on Vtg through this experiment could provide the basis of an immunological assay for sex identification and maturational status of the Giant grouper. Consequently, the aim of the present study was to establish a simple, efficient and non-lethal technique by using Vtg as biomarker indicator for rapid sex identification without killing or seriously injuring the sampled individuals.