alexa Using 3D High-Content Analysis and Epigenetic Phenotyping of Cells in the Characterization of Human Prostate Tissue Heterogeneity
ISSN: 2168-9431

Single Cell Biology
Open Access

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Using 3D High-Content Analysis and Epigenetic Phenotyping of Cells in the Characterization of Human Prostate Tissue Heterogeneity

Jian Tajbakhsh*and Kolja Wawrowsky
Translational Cytomics Group, Cedars-Sinai Medical Center, Los Angeles, CA, USA
Corresponding Author : Jian Tajbakhsh
Translational Cytomics Group
Cedars-Sinai Medical Center
Los Angeles, CA, USA
Tel: +1 310-423-3277
E-mail:[email protected]
Received March 27, 2015; Accepted March 30, 2015; Published March 31, 2015
Citation: Tajbakhsh J and Wawrowsky K (2015) Using 3D High-Content Analysis and Epigenetic Phenotyping of Cells in the Characterization of Human Prostate Tissue Heterogeneity. Single Cell Biol 4:i104. doi: 10.4172/2168-9431.1000i104
Copyright: ©2015 Tajbakhsh J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Next-generation 3D high-content imaging and analysis enables the quantification and display of tissue heterogeneity regarding subcellular properties, and hence will improve the diagnostic characterization of biopsied tissues. ( Upper left) Needle-biopsy section of human prostate adenocarcinoma, immuno-labeled for global DNA methylation (5methylcytosine, false-colored green) and counterstained for global nuclear DNA with 4',6-diamidino-2-phenylindole (DAPI, false-colored blue) and imaged with a 20X NA=0.7 lens.

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