alexa Using Nano-Arrayed Structures in Sol-Gel Derived Mn2+ D
ISSN: 2155-6210

Journal of Biosensors & Bioelectronics
Open Access

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Research Article

Using Nano-Arrayed Structures in Sol-Gel Derived Mn2+ Doped Tio2 for High Sensitivity Urea Biosensor

Ravi R. Pandey1, Krishan K. Saini2 and Marshal Dhayal1*

1Center for Cellular and Molecular Biology, Uppal Road, Hyderabad, India

2National Physical Laboratory, Dr KS Krinan Marg, New Delhi, India

*Corresponding Author:
Dr. Marshal Dhayal
Center for Cellular and Molecular Biology
Uppal Road, Hyderabad India
Tel: +91-040-27192520
Fax: +91-040- 271-60591
E-mail: [email protected]

Received Date: August 06, 2010; Accepted Date: August 25, 2010; Published Date: August 25, 2010

Citation: Pandey RR, Saini KK, Dhayal M (2010) Using Nano-Arrayed Structures in Sol-Gel Derived Mn2+ Doped Tio2 for High Sensitivity Urea Biosensor. Journal of Biosensors & Bioelectron 1:101 doi:10.4172/2155-6210.1000101

Copyright: © 2010 Pandey RR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Ordered and self-organized nano-array structures have been developed by Mn 2+ doping in TiO 2 thin fi lms deposition on conducting substrates by dip coating technique. Mn doped TiO 2 thin fi lms exhibits better bioactivity for enzyme immobilization and cyclic voltammetry measurement has been used for qualitative characterization of electrochemical induction of oxidation-reduction process in TiO 2 and Mn doped TiO 2 fi lms. Due to presence of Mn 2+ ions at fi lm surface, current voltage characteristic of Mn doped TiO 2 matrix was enhanced by a factor of ten and it had also reduced the crystallite size and promoted transformation of anatase to rutile phase of TiO 2 . Urea concentration in the electrolyte was determined by observing chronoamperometry response on urease immobilized working electrodes. The urea detection sensitivity of the Mn doped TiO 2 thin fi lms base platform was 2.3 μ A mM − 1 cm − 2 which is about 15 times higher from only TiO 2 base platforms. Such kind of enzyme–TiO 2 /Mn nano-array electrode could contribute a potential prospect in low cost biomedical diagnosis.

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