alexa Using of Coffee and Cardamom Mixture to Ameliorate Oxidative Stress Induced in γ-irradiated Rats
ISSN: 2161-1009

Biochemistry & Analytical Biochemistry
Open Access

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Research Article

Using of Coffee and Cardamom Mixture to Ameliorate Oxidative Stress Induced in γ-irradiated Rats

Refaat G. Hamzaa* and Nadia N. Osman

Department of food irradiation, Atomic Energy Authority, Cairo, Egypt

*Corresponding Author:
Refaat G. Hamzaa
Department of Food Irradiation
Atomic Energy Authority, 3 Ahmed El Zomor St.
El Zohour District, 8th District, Nasr City, Cairo
Tel: +201224109349
Fax: +20222749298

Received Date: July 06 2012; Accepted Date: July 19, 2012; Published Date: July 30, 2012

Citation: Hamzaa RG, Osman NN (2012) Using of Coffee and Cardamom Mixture to Ameliorate Oxidative Stress Induced in γ-irradiated Rats. Biochem Anal Biochem 1:113. doi: 10.4172/2161-1009.1000113

Copyright: © 2012 Hamzaa RG, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Human exposure to ionizing radiation induced overproduction of free radicals leading to oxidative stress. This study aims to evaluate the possibility of using of coffee and cardamom mixture, as natural antioxidant compounds, to ameliorate the damage effect of oxidative stress. Phenolic contents in coffee and essential oils in cardamom were identified by using HPLC chromatography and GC/MS analysis. Four groups of adult male rats were used; the control group (A), the second group (B) received orally mixture extract of coffee and cardamom (60 mg/100g body weight) for 8 weeks, the third group (C) γ-irradiated (6 GY) and the fourth group (D) received orally mixture extract for 8 weeks and exposed to γ-irradiation at the 4th week. The results revealed that the administration of mixture extract of coffee and cardamom were significantly reduced the damage effect induced by γ-irradiation exposure. Accordingly, it could be concluded that, via the adjustment of the antioxidant status, decreasing the releasing of lipid peroxides and the subsequent amending of different biochemical parameters and some hormones, mixture of coffee and cardamom could ameliorate the hazardous effects of oxidative stress induced by irradiation exposure.


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