Utility of Next-generation sequencing in Managing Bacterial Vaginosis: Examples from Clinical PracticeBostwick GD1*, Hunt CA2, Parker LR3, Daniel Mcdyer4, Woody J1 and Budd W1
- *Corresponding Author:
- David G Bostwick
Chief Medical Officer, Granger Diagnostics
601 Biotech Drive, North Chesterfield
E-mail: [email protected]
Received date: June 27, 2016; Accepted date: July 26, 2016; Published date: August 10, 2016
Citation: Bostwick GD, Hunt CA, Parker LR, Daniel Mcdyer, Woody J, et al (2016) Utility of Next-generation sequencing in Managing Bacterial Vaginosis: Examples from Clinical Practice. J Women's Health Care 5:322. doi: 10.4172/2167-0420.1000322
Copyright: © 2016 Bostwick GD et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
There is an unacceptably high recurrence rate for bacterial vaginosis and vaginitis owing to multiple causes, including misdiagnosis and empirical under-treatment and over-treatment. A diagnostic test that provided complete knowledge of the vaginal microbiome in patients with bacterial vaginosis (BV) would fill an important void. In initial evaluations of Next-generation Sequencing (NGS), this new test offers great promise.As a clinical diagnostic for primary or recurrent bacterial vaginosis, NGS by simple vaginal swab offers heightened sensitivity and specificity compared to culture or limited three-organism genetic tests. NGS holds valuable potential in multiple BV quandary situations, including: (a) Recurrent BV in a patient that failed initial therapy, (b) Symptoms of BV in a patient with false-positive Amsel criteria, (c) Gardnerellavaginalis-negative BV with mixed infection. Multiplex next-generation sequencing (NGS) provides a complete and accurate description of the composition of the complex polymicrobial vaginal microbiome and associated antimicrobial resistance-determining genes, facilitating personalized diagnosis and therapy for patients with bacterial vaginosis, STIs and vaginitis.