alexa Utilization of Agro Residue Corncob for Production of Acetone-Butanol Using Clostridium acetobutylicum and Process Optimization through RSM
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
Open Access

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Research Article

Utilization of Agro Residue Corncob for Production of Acetone-Butanol Using Clostridium acetobutylicum and Process Optimization through RSM

Mahendra Kumar*, Dilip Kumar and Brajesh Singh

Department of Biochemical Engineering and Food technology, Harcourt Butler Technological Institute (HBTI), Uttar Pradesh, India

*Corresponding Author:
Mahendra Kumar
Contractual Lecturer
Department of Biochemical Engineering and Food technology
Harcourt Butler Technological Institute (HBTI)
Kanpur-208002, Uttar Pradesh, India
Tel: +91-8115890961
E-mail: [email protected]

Published date: September 16, 2014; Published date: October 07, 2014; Published date: October 14, 2014

Citation: Kumar M, Kumar D, Singh B (2014) Utilization of Agro Residue Corncob for Production of Acetone-Butanol Using Clostridium acetobutylicum and Process Optimization through RSM. J Microb Biochem Technol S8:005. doi:10.4172/1948-5948.S8-005

Copyright: © 2014 Kumar M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited



Acetone-Butanol-Ethanol (ABE) fermentation is an important industrial process for acetone and butanol production. In early 1950 due to the rise of cheaper petrochemical synthesis and increased cost of fermentation raw materials, these are predominately produced through chemical synthesis which relies on crude oil supply. With the growing concerns of environmental issues, depleting fossil resources and increasing crude oil price, interest has returned to fermentative production, not only as a chemical but also as an alternative biofuel. To overcome the limitations of conventional ABE fermentation such as low titer and high substrate cost emphases areas under research are utilization of renewable and low-cost feedstocks, development of novel fermentation processes, alternative product recovery technologies and metabolic engineering of solvent-producing microorganisms. In the present study the rotatable central composite design and response surface technique that was employed for optimization work in many studies is successfully used. The optimum conditions for acetone production of corncob were found as temperature 36.71°C, pH 4.16 and sugar (g/l) 99.94, butanol production of corncob were found as temperature 35.44°C, pH 4.79 and sugar (g/l) 91.96.


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