Utilization of Multiphoton Imaging For Real-Time Fate Determination of Mesenchymal Stem Cells in an Immunocompetent Mouse ModelJay T Myers1, Agne Petrosiute1,2 and Alex Y Huang1,2*
- *Corresponding Author:
- Alex Y Huang, M.D., Ph.D.
Department of Pediatrics, Case Western Reserve University
WRB 6528, 2103 Cornell Road, Cleveland, OH 44106-7288, USA
E-mail: [email protected]
Received date: June 20, 2014; Accepted date: July 17, 2014; Published date: July 19, 2014
Citation: Myers JT, Petrosiute A, Huang AY (2014) Utilization of Multiphoton Imaging For Real-Time Fate Determination of Mesenchymal Stem Cells in an Immunocompetent Mouse Model. J Stem Cell Res Ther 4:217. doi:10.4172/2157-7633.1000217
Copyright: © 2014 Myers JT, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The clinical application of Mesenchymal Stem Cells (MSCs) for the treatment of a variety of diseases is the focus of intense research. Despite large research efforts many questions regarding MSC biology in vivo remain unanswered. For instance, we do not know for certain whether MSCs exert their therapeutic effects directly within the target tissue or indirectly by influencing the polarization of other cell types, such as macrophages, which can then home to the target tissue microenvironment. To help address this issue, the application of intravital multiphoton microscopy allows for the determination of the dynamic action of intact MSCs versus endogenous host cells at the target tissue site in real time.