alexa Utilization of Multiphoton Imaging For Real-Time Fate Determination of Mesenchymal Stem Cells in an Immunocompetent Mouse Model | OMICS International | Abstract
ISSN: 2157-7633

Journal of Stem Cell Research & Therapy
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Commentary

Utilization of Multiphoton Imaging For Real-Time Fate Determination of Mesenchymal Stem Cells in an Immunocompetent Mouse Model

Jay T Myers1, Agne Petrosiute1,2 and Alex Y Huang1,2*

1Department of Pediatrics, Case Western Reserve University, Cleveland, Ohio 44106, USA

2Angie Fowler AYA Cancer Institute, University Hospitals Case Medical Center / Rainbow Babies & Children’s Hospital, Cleveland, Ohio 44106, USA

*Corresponding Author:
Alex Y Huang, M.D., Ph.D.
Department of Pediatrics, Case Western Reserve University
WRB 6528, 2103 Cornell Road, Cleveland, OH 44106-7288, USA
Tel: 1-216-368-1271
Fax: 1-216-844-5431
E-mail: [email protected]

Received date: June 20, 2014; Accepted date: July 17, 2014; Published date: July 19, 2014

Citation: Myers JT, Petrosiute A, Huang AY (2014) Utilization of Multiphoton Imaging For Real-Time Fate Determination of Mesenchymal Stem Cells in an Immunocompetent Mouse Model. J Stem Cell Res Ther 4:217. doi:10.4172/2157-7633.1000217

Copyright: © 2014 Myers JT, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The clinical application of Mesenchymal Stem Cells (MSCs) for the treatment of a variety of diseases is the focus of intense research. Despite large research efforts many questions regarding MSC biology in vivo remain unanswered. For instance, we do not know for certain whether MSCs exert their therapeutic effects directly within the target tissue or indirectly by influencing the polarization of other cell types, such as macrophages, which can then home to the target tissue microenvironment. To help address this issue, the application of intravital multiphoton microscopy allows for the determination of the dynamic action of intact MSCs versus endogenous host cells at the target tissue site in real time.

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