alexa UV - Spectrophotometric Method Development and Validati
e-ISSN: 2320-0812 p-ISSN: 2347-2340

Research & Reviews: Journal of Pharmaceutical Analysis
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Research Article

UV - Spectrophotometric Method Development and Validation for Determination of Raloxifene in Pharmaceutical Dosage Form.

Nagaraju PT, K Venu Gopal, Murali Krishna NV*, Bhargavi V, and Srinivasulu N

Department of Pharmaceutical Analysis, Nirmala College of Pharmacy, Kadapa - 516002, Andhra Pradesh, India.

*Corresponding Author:
Murali Krishna NV ,
Department of Pharmaceutical Analysis,
Nirmala College of Pharmacy, Kadapa - 516002,
Andhra Pradesh, India.
Tel: +91 9490134263

Received: 04 August 2014 Accepted: 22 September 2014

 

Abstract

A simple, precise and economical second order derivative method has been developed for the estimation of Raloxifene in bulk and pharmaceutical formulations. In this method Raloxifene showed sharp peak at 288 nm when n= 1 and linearity was measured at 288 nm. It obeyed Beer’s law in the concentration range of 2-12 mcg/ml. The LOD and LOQ were found to be 0.32 mcg/ml and 0.98 mcg/ml respectively. A recovery of Raloxifene in tablet formulation was observed in the range of 99.38-100.60%. The proposed method is precise, accurate and reproducible and can be extended to the analysis of Raloxifene in bulk and pharmaceutical formulations.

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