UV-Spectrophotometric-Assisted Chemometric Methods for the Simultaneous Determination of Metformin Hydrochloride and Gliclazide in Pharmaceutical Formulations
- *Corresponding Author:
- Rahul Bhaskar
Department of Pharmacy
Mahatma Jyoti Rao Phoole University, Jaipur, India
E-mail: [email protected]
Received date: May 18, 2012; Accepted date: June 25, 2012; Published date: June 27, 2012
Citation: Bhaskar R, Bhaskar R, Sagar MK, Saini V, Bhat KM (2012) UVSpectrophotometric- Assisted Chemometric Methods for the Simultaneous Determination of Metformin Hydrochloride and Gliclazide in Pharmaceutical Formulations. Pharmaceut Anal Acta 3:158. doi: 10.4172/2153-2435.1000158
Copyright: © 2012 Bhaskar R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In this study, the simultaneous determination of Metformin hydrochloride (MET) and Gliclazide (GLZ) in pharmaceuticals by chemometric approaches using UV spectrophotometry has been reported. Spectra of MET and GLZ were recorded at several concentrations within their linear ranges between wavelengths of 200 nm to 400 nm in pH 6.8 phosphate buffer. Partial Least Squares regression (PLS) and Net Aanalyte Preprocessing combined with Classical Least Square (NAP/CLS) were used for chemometric analysis of data and the parameters of the chemometric procedures were optimized. The recoveries were satisfactory and statistically comparable. The method was successfully applied to pharmaceutical formulation, tablet, with no interference with excipients as indicated by the recovery study results. The proposed methods are simple, rapid and can be easily used in the quality control of drugs as alternative analysis tools.