Validated and Sensitive Spectrophotometric Method for the Determination of Amitriptyline Hydrochloride
Deepakumari HN, Prashanth MK, Revanasiddappa HD*
Department of Chemistry, University of Mysore, Manasagangothri, Mysore-570 006, India
- Corresponding Author:
- Revanasiddappa HD
Department of Chemistry, University of Mysore
Manasagangothri, Mysore-570 006, India
E-mail: [email protected]
Received date: July 24, 2013; Accepted date: September 02, 2013; Published date: September 09, 2013
Citation: Deepakumari HN, Prashanth MK, Revanasiddappa HD (2013) Validated and Sensitive Spectrophotometric Method for the Determination of Amitriptyline Hydrochloride. Chem Sci J 4:072. doi:10.4172/2150-3494.1000072
Copyright: © 2013 Deepakumari HN, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A simple, rapid, selective and highly sensitive spectrophotometric method is described for the quantitative determination of a tricyclic antidepressant drug, amitriptyline hydrochloride (AMT) in pure and in pharmaceutical preparations. The method is based on the bromination of AMT with known excess of bromine. The unreacted bromine is determined based on its ability to bleach the dye Eiochrome blue black R quantitatively at 530 nm. Beer’s law was obeyed over the concentration range 0.0–15 µg/mL. The molar absorptivity value was found to be 1.345×104 L/moL/cm, with the corresponding Sandell’s sensitivity values of 0.0233 µg/cm2. The limits of detection and (LOD) and quantification (LOQ) are also reported for the developed method. Intra- and inter-assay precision and accuracy of the method was established according to the current ICH guidelines. Applications of the procedure to the analysis of various pharmaceutical preparations gave reproducible and accurate results. Further, No interferences were observed from excipients and the validity of the method was tested against reference method. Percent of relative recoveries values were 98.57% to 100.52%.