alexa Validated HPLC and Thin Layer-Densitometric Methods for
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

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Research Article

Validated HPLC and Thin Layer-Densitometric Methods for Determination of Quetiapine Fumarate in Presence of its Related Compounds

Sawsan MA1, Hesham S2, Marianne N1,3 and El-Maraghy MC4*

1Analytical Chemistry department, Faculty of Pharmacy, Cairo University, Kasr-El Aini Street, 11562Cairo, Egypt

2Pharmaceutical Analytical Chemistry department, Faculty of Pharmacy, Deraya University, Minia, Egypt

3Pharmaceutical Analytical Chemistry department, Faculty of Pharmacy & Drug Technology, Heliopolis University, 3 Cairo Belbeis desert road, 2834El- Horria, Cairo, Egypt

4Analytical Chemistry department, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), 11787 6th October city, Egypt

*Corresponding Author:
Christine M. El-Maraghy
Analytical Chemistry Department
Faculty of Pharmacy
October University for Modern Sciences and Arts (MSA)
11787 6th October City, Egypt
Tel:+20-01223553561
E-mail: [email protected]

Received date: June 22, 2015; Accepted date: June 30, 2015; Published date: July 07, 2015

Citation: Sawsan MA, Hesham S, Marianne N, El-Maraghy MC (2015) Validated HPLC and Thin Layer-Densitometric Methods for Determination of Quetiapine Fumarate in Presence of its Related Compounds. J Chromatogr Sep Tech 6:279. doi:10.4172/2157-7064.1000279

Copyright: © 2015 Sawsan MA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Two chromatographic methods were developed for determination of quetiapine fumarate in presence of three related compounds; namely quetiapine N-oxide, des-ethanol quetiapine and quetiapine lactam, in pure form and pharmaceutical preparation. The first method depended on densitometric thin layer chromatography where the separation was achieved using silica gel 60F(254) plates as stationary phase and toluene:1,4-dioxane:dimethylamine (5:8:2, v/v/v) as a mobile phase. The second method utilized the reverse phase high performance liquid chromatographic technique, using C18 column and methanol: acetonitrile: phosphate buffer (pH 5.3) in a ratio (19:40:41, v/v/v) as a mobile phase. The flow rate was1 mL min-1 and UV-detection was at wavelength 220 nm. The validation parameters of the developed methods were calculated and the results obtained were statistically compared with those of the HPLC manufacturer method.

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