Validated HPLC and Thin Layer-Densitometric Methods for Determination of Quetiapine Fumarate in Presence of its Related Compounds
- *Corresponding Author:
- Christine M. El-Maraghy
Analytical Chemistry Department
Faculty of Pharmacy
October University for Modern Sciences and Arts (MSA)
11787 6th October City, Egypt
E-mail: [email protected]
Received date: June 22, 2015; Accepted date: June 30, 2015; Published date: July 07, 2015
Citation: Sawsan MA, Hesham S, Marianne N, El-Maraghy MC (2015) Validated HPLC and Thin Layer-Densitometric Methods for Determination of Quetiapine Fumarate in Presence of its Related Compounds. J Chromatogr Sep Tech 6:279. doi:10.4172/2157-7064.1000279
Copyright: © 2015 Sawsan MA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Two chromatographic methods were developed for determination of quetiapine fumarate in presence of three related compounds; namely quetiapine N-oxide, des-ethanol quetiapine and quetiapine lactam, in pure form and pharmaceutical preparation. The first method depended on densitometric thin layer chromatography where the separation was achieved using silica gel 60F(254) plates as stationary phase and toluene:1,4-dioxane:dimethylamine (5:8:2, v/v/v) as a mobile phase. The second method utilized the reverse phase high performance liquid chromatographic technique, using C18 column and methanol: acetonitrile: phosphate buffer (pH 5.3) in a ratio (19:40:41, v/v/v) as a mobile phase. The flow rate was1 mL min-1 and UV-detection was at wavelength 220 nm. The validation parameters of the developed methods were calculated and the results obtained were statistically compared with those of the HPLC manufacturer method.