alexa Validation of an Experimental Animal Model for Corneal Additive Surgery
ISSN: 2155-9570

Journal of Clinical & Experimental Ophthalmology
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Research Article

Validation of an Experimental Animal Model for Corneal Additive Surgery

Lucía Ibares-Frías1-3*, Patricia Gallego1,4, Roberto Cantalapiedra-Rodriguez4, Maria Cruz Valsero1,5 , Santiago Mar1, Jesús Merayo-Lloves1,6 and Maria Carmen Martínez-García1,4
1Group of Optical Diagnostic Techniques, University of Valladolid, Valladolid, Spain
2Instituto de Oftalmobiología Aplicada (IOBA), University of Valladolid, Valladolid, Spain
3Ophthalmology Department, Hospital Clínico Universitario de Valladolid, Valladolid, Spain
4Cell Biology, Histology and Pharmacology Department, University of Valladolid, Valladolid, Spain
5Biostatistics Department, University of Valladolid, Valladolid, Spain
6Fundación de Investigación Oftalmológica, Instituto Oftalmológico Fernandez Vega, Oviedo, Spain
Corresponding Author : Lucía Ibares-Frías
Departamento de Histología
Biología Celular y Farmacología
Facultad de Ciencias de la Salud
Universidad de Valladolid
Avenida Ramón y Cajal 7 47003, Valladolid, Spain
Tel: 0034658944269
Email: [email protected]
Received August 01, 2014; Accepted September 26, 2014,; Published September 30, 2014
Citation: Ibares-Frías L, Gallego P, Cantalapiedra-Rodriguez R, Valsero MC, Mar S (2014) Validation of an Experimental Animal Model for Corneal Additive Surgery. J Clin Exp Ophthalmol 5:360. doi: 10.4172/2155-9570.1000360
Copyright: © 2014 Ibares-Frías L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

Purpose: To assess the hen cornea as a model for training and future wound healing studies after implantation of intrastromal corneal ring segments (ICRS) by clinical and optical outcomes. Setting: University of Valladolid, Valladolid, Spain. Design: Experimental study. Methods: One 90°, 150-μm thick polymethyl methacrylate Ferrara ICRS segment was manually implanted at 70-80% depth of 192 Gallus domesticus corneas. Clinical follow-up for 6 months included monitoring corneal thickness, epithelial wound closure, edema, haze, and the location and severity of deposits. The refractive state was also measured. After each animal was euthanized, corneas were processed for direct transmittance and histological analysis. Results: Complications were present in 16% of the eyes. Epithelial wound closure was completed at 3 ± 2 days. A slight corneal edema in the channel site was present for the first 15 days. All corneas had deposits by 4 months located along the inner, outer curvatures and under the segments. Corneal haze was present only at the incision site. ICRS induced hyperopic changes in the refractive state without changes in direct transmitance of central cornea. New cells and extracellular matrix were present around the segment where deposits were seen on clinical follow-up. Conclusions: With hen as an animal model, ICRS were implanted in a precise and reproducible way after a learning curve. Similar to humans, the follow-up period during the first 6 months after implantation showed fast wound closure, deposits, and haze at the incision site. ICRS in hens also reduced the refractive power without affecting the central cornea.

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